Burtscher Wesley P, List Marna A, Payton Adam C, McDaniel Stuart F, Carey Sarah B
Department of Biology University of Florida Gainesville Florida 32611 USA.
Appl Plant Sci. 2021 Apr 30;9(4):e11418. doi: 10.1002/aps3.11418. eCollection 2021 Apr.
Mosses have long served as models for studying many areas of plant biology. Investigators have used two-dimensional measurements of juvenile growth from photographs as a surrogate for dry-weight biomass. The relationship between area and biomass, however, has not been critically evaluated.
Here we grew axenic tissue cultures of 10 isolates to study the relationship between these parameters. We measured area and biomass on replicate cultures with two distinct starting inoculum sizes each week for three weeks. We then examined the correlation between area and biomass as well as the influence of variation in inoculum size on both parameters.
We found a strong correlation between area and biomass after two weeks of growth. Furthermore, we found inoculum size affected biomass during the first week of growth but not in subsequent weeks and inoculum size had no detectable effect on area.
These analyses provide experimental confirmation that area is a suitable proxy for biomass and provide clear guidelines for when inoculum size variation may affect downstream growth estimates.
长期以来，苔藓一直是研究植物生物学许多领域的模型。研究人员使用从照片中获取的二维幼体生长测量数据作为干重生物量的替代指标。然而，面积与生物量之间的关系尚未得到严格评估。
在此，我们培养了10个分离株的无菌组织培养物，以研究这些参数之间的关系。我们在每周有两种不同起始接种量的重复培养物上测量面积和生物量，持续三周。然后，我们研究了面积与生物量之间的相关性，以及接种量变化对这两个参数的影响。
我们发现生长两周后面积与生物量之间存在很强的相关性。此外，我们发现接种量在生长的第一周影响生物量，但在随后几周没有影响，并且接种量对面积没有可检测到的影响。
这些分析提供了实验证实，面积是生物量的合适替代指标，并为接种量变化何时可能影响下游生长估计提供了明确的指导方针。
