G3BP1在人前列腺良恶性组织中的表达
Expression of G3BP1 in benign and malignant human prostate tissues.
作者信息
Wang Chao, Cui Qiqi, Du Runxuan, Liu Shuang, Tian Shaoping, Huang Hua, Jiang Yihua, Chen Ruibing, Niu Yuanjie, Yue Dan, Wang Yong
机构信息
Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology and School of Medical Laboratory, Tianjin Medical University, Tianjin, China.
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
出版信息
Transl Androl Urol. 2021 Apr;10(4):1665-1675. doi: 10.21037/tau-20-1450.
BACKGROUND
Prostate cancer (PCa) is the world's leading type of cancer in men. GTPase-activating protein SH3 domain-binding protein 1 (G3BP1) is overexpressed in a variety of tumors. However, there are limited studies in PCa concerning G3BP1. This present study was to investigates the expression of G3BP1 and the mechanism of action on PCa.
METHODS
We explored the G3BP1 expression in PCa using the TCGA database and verified it using clinical samples by immunohistochemistry (IHC) methods. G3BP1 and Androgen receptor (AR) status of 104 human PCa and 50 benign prostate hyperplasia (BPH) samples were analyzed by IHC and the association between G3BP1 expression and biochemical recurrence was determined. Moreover, we generated G3BP1 knockdown cell lines in human PCa LNCaP cell lines, to observe AR changes.
RESULTS
G3BP1 and AR were overexpressed in PCa compared to BPH tissues. The expression of G3BP1 and AR was positively correlated with the malignant degree of the tumor. Higher G3BP1 expression showed a trend toward biochemical recurrence. Western blot showed downregulation of G3BP1 affected AR expression levels.
CONCLUSIONS
Our study suggested that G3BP1 was frequently upregulated in PCa and closely related to AR expression and tumor metastasis. Besides, G3BP1 might be associated with biochemical recurrence. These results supply potential target for the management of the PCa.
背景
前列腺癌（PCa）是全球男性中最主要的癌症类型。GTP酶激活蛋白SH3结构域结合蛋白1（G3BP1）在多种肿瘤中过度表达。然而，关于G3BP1在前列腺癌中的研究有限。本研究旨在探讨G3BP1的表达及其在前列腺癌中的作用机制。
方法
我们利用TCGA数据库探究前列腺癌中G3BP1的表达，并通过免疫组织化学（IHC）方法用临床样本进行验证。通过IHC分析104例人前列腺癌和50例良性前列腺增生（BPH）样本的G3BP1和雄激素受体（AR）状态，并确定G3BP1表达与生化复发之间的关联。此外，我们在人前列腺癌LNCaP细胞系中构建G3BP1敲低细胞系，以观察AR的变化。
结果
与BPH组织相比，G3BP1和AR在前列腺癌中过度表达。G3BP1和AR的表达与肿瘤的恶性程度呈正相关。较高的G3BP1表达显示出生化复发的趋势。蛋白质免疫印迹显示G3BP1的下调影响AR表达水平。
结论
我们的研究表明，G3BP1在前列腺癌中经常上调，与AR表达和肿瘤转移密切相关。此外，G3BP1可能与生化复发有关。这些结果为前列腺癌的治疗提供了潜在靶点。
Zotero 插件