豚鼠脂蛋白脂肪酶的组织特异性调节；营养状态和肿瘤坏死因子对脂肪组织、心脏和肝脏中mRNA水平的影响。

Tissue-specific regulation of guinea pig lipoprotein lipase; effects of nutritional state and of tumor necrosis factor on mRNA levels in adipose tissue, heart and liver.

作者信息

Enerbäck S, Semb H, Tavernier J, Bjursell G, Olivecrona T

机构信息

Department of Medical Biochemistry, University of Gothenburg, Sweden.

出版信息

Gene. 1988 Apr 15;64(1):97-106. doi: 10.1016/0378-1119(88)90484-2.

DOI:10.1016/0378-1119(88)90484-2

PMID:3396878

Abstract

Levels of mRNA for lipoprotein lipase (LPL) in guinea pig epididymal adipose tissue, heart and liver were determined by dot blot analysis of total RNA using a cDNA probe complementary to the coding region, and compared to the LPL activity. For adipose tissue we also measured the incorporation of radioactivity into immunoprecipitable LPL after pulse-labeling with [35S]methionine. LPL activity was 93%, LPL mRNA 82% and LPL synthesis 85% lower in epididymal fat pads from animals fasted for 48 h compared to rigorously fed animals. In contrast, neither LPL activity nor LPL mRNA levels differed in heart. A single dose of tumor necrosis factor (TNF) decreased LPL activity and LPL mRNA in fat pads with no effects in heart. In the liver, TNF caused a marked increase in LPL mRNA levels, which are normally very low. Northern-blot analysis confirmed a previous observation that the patterns of mRNA species differ between heart, in which a 3.8-kb mRNA dominates, and adipose tissue, in which the LPL mRNAs of 3.3 and 2.1 kb occur in similar abundance as the 3.8-kb species.

摘要

通过使用与编码区互补的cDNA探针，对豚鼠附睾脂肪组织、心脏和肝脏中的总RNA进行斑点印迹分析，测定脂蛋白脂肪酶（LPL）的mRNA水平，并与LPL活性进行比较。对于脂肪组织，我们还在用[35S]甲硫氨酸脉冲标记后，测量了放射性掺入可免疫沉淀的LPL中的情况。与严格喂食的动物相比，禁食48小时的动物附睾脂肪垫中的LPL活性降低了93%，LPL mRNA降低了82%，LPL合成降低了85%。相比之下，心脏中的LPL活性和LPL mRNA水平均无差异。单剂量的肿瘤坏死因子（TNF）降低了脂肪垫中的LPL活性和LPL mRNA，对心脏无影响。在肝脏中，TNF导致LPL mRNA水平显著升高，而肝脏中的LPL mRNA水平通常非常低。Northern印迹分析证实了先前的观察结果，即心脏和脂肪组织中mRNA种类的模式不同，心脏中以3.8 kb的mRNA为主，而脂肪组织中3.3 kb和2.1 kb的LPL mRNA与3.8 kb的种类丰度相似。