盘基网柄菌质粒核定位的建立。

Establishment of the nuclear location of Dictyostelium discoideum plasmids.

作者信息

Ashktorab H, Welker D L

机构信息

Department of Biology, Utah State University, Logan 84322-5500.

出版信息

Gene. 1988 May 15;65(1):41-9. doi: 10.1016/0378-1119(88)90415-5.

DOI:10.1016/0378-1119(88)90415-5

PMID:3396881

Abstract

The nuclear location of the Dictyostelium discoideum plasmids was studied using a biochemical approach based on the presence of plasmid sequences in nucleosomes. This analysis revealed that all four of the known plasmids (Ddp1, Ddp2, Ddp3, Ddp5) are present in chromatin. This evidence establishes that the D. discoideum plasmids are not cytoplasmic but located in the nucleus. D. discoideum is unique among eukaryotes in possessing a group of nonhomologous endogenous plasmids in its nucleus. These plasmids are excellent starting material for construction of nuclear transformation and expression vectors. Such vectors upon transformation into D. discoideum are also present in chromatin as expected for DNA located in the nucleus.

摘要

利用基于核小体中质粒序列存在情况的生化方法，对盘基网柄菌质粒的核定位进行了研究。该分析表明，所有四种已知质粒（Ddp1、Ddp2、Ddp3、Ddp5）都存在于染色质中。这一证据证实，盘基网柄菌质粒并非存在于细胞质中，而是位于细胞核内。盘基网柄菌在真核生物中独一无二，其细胞核中拥有一组非同源的内源质粒。这些质粒是构建核转化和表达载体的优质起始材料。转化到盘基网柄菌中的此类载体同样存在于染色质中，正如位于细胞核中的DNA所预期的那样。