印度尼西亚慢性乙型肝炎患者维生素D受体多态性的基因型分布

Genotype Distribution of Vitamin D Receptor Polymorphisms among Indonesian Patients with Chronic Hepatitis B.

作者信息

Arfianti Arfianti, Sabillah Sumpena Athalah, Andrini Djojosugito Fauzia, Kartika Sari Dita, Julia Paulina Ariza

机构信息

Department of Medical Biology, Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia.

Undergraduate of Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia.

出版信息

Rep Biochem Mol Biol. 2021 Jan;9(4):463-469. doi: 10.52547/rbmb.9.4.463.

DOI:10.52547/rbmb.9.4.463

PMID:33969140

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8068445/

Abstract

BACKGROUND

Chronic hepatitis B is a necro-inflammatory of the liver parenchyma caused by hepatitis B virus (HBV) infection leading to liver cirrhosis and hepatocellular carcinoma (HCC). Genetic variants including single nucleotide polymorphisms (SNPs) within genes regulating immune response may contribute to the progression of chronic hepatitis B (CHB) infection. This study aimed to examine the genotype distribution of vitamin D receptor (VDR) polymorphism among patients with CHB infection and to study its association with the development of cirrhosis and hepatoma.

METHODS

This cross-sectional study analysed 75 CHB patients, consisting of 36 CHB patients without cirrhosis, 25 CHB patients with cirrhosis, and 14 CHB patients with hepatoma. VDR polymorphism was examined using the Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) method.

RESULTS

Alanine aminotransferase (ALT) and alpha fetoprotein (AFP) levels did not show any significant differences between study groups, but albumin levels in CHB patients with cirrhosis and hepatoma were significantly lower than CHB patients without cirrhosis (p< 0.05). In contrast, the bilirubin levels in CHB patients with cirrhosis was higher than in CHB patients' cirrhosis. The most common genotypes of VDR polymorphisms were Ff (57.3%), TT (72%), aa (48%) and bb (74.7%) for Fok1, Taq1, Apa1 and Bsm1 respectively. There was no significant different in the genotype distribution of VDR polymorphism between CHB patients without cirrhosis and CHB with cirrhosis or hepatoma.

CONCLUSION

This study suggest that VDR gene polymorphism may not contribute to the progression of CHB infection.

摘要

背景

慢性乙型肝炎是由乙型肝炎病毒（HBV）感染引起的肝实质坏死性炎症，可导致肝硬化和肝细胞癌（HCC）。包括调节免疫反应基因内的单核苷酸多态性（SNP）在内的基因变异可能有助于慢性乙型肝炎（CHB）感染的进展。本研究旨在检测CHB感染患者中维生素D受体（VDR）多态性的基因型分布，并研究其与肝硬化和肝癌发生的关系。

方法

本横断面研究分析了75例CHB患者，包括36例无肝硬化的CHB患者、25例肝硬化的CHB患者和14例肝癌的CHB患者。采用扩增阻滞突变系统聚合酶链反应（ARMS-PCR）方法检测VDR多态性。

结果

各研究组间丙氨酸氨基转移酶（ALT）和甲胎蛋白（AFP）水平无显著差异，但肝硬化和肝癌的CHB患者白蛋白水平显著低于无肝硬化的CHB患者（p<0.05）。相比之下，肝硬化的CHB患者胆红素水平高于无肝硬化的CHB患者。VDR多态性最常见的基因型分别为Fok1的Ff（57.3%）、Taq1的TT（72%）、Apa1的aa（48%）和Bsm1的bb（74.7%）。无肝硬化的CHB患者与有肝硬化或肝癌CHB患者的VDR多态性基因型分布无显著差异。