印度犬只中犬腺病毒2型的分子特征分析

Molecular characterization of canine adenovirus type 2 in dogs from India.

作者信息

Raja P, Sachin V, Parthiban M, Janaki P Aishwarya

机构信息

Department of Animal Biotechnology, Faculty of Basic Sciences, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600007 India.

Hindustan Institute of Technology and Science, Chennai, 603103 India.

出版信息

Virusdisease. 2021 Jun;32(2):369-374. doi: 10.1007/s13337-021-00690-7. Epub 2021 May 5.

DOI:10.1007/s13337-021-00690-7

PMID:33969151

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8096630/

Abstract

A total of 26 nasal swab samples were collected from dogs with gastroenteritis and respiratory tract infections in and around Chennai, India during 2019-20. All the samples were subjected to PCR using common primers for rapid diagnosis and differentiation of CAV1 and CAV2. Only one sample produced an amplicon of 1030 bp indicating the presence of CAV2 which was confirmed by further sequencing. The analysis of the sequence revealed 100 per cent identity with other CAV type 2 isolates from Brazilian, Canadian and USA strains and 95.9 per cent identity with other Indian CAV2 strains. The phylogenetic analysis of E3 gene reveal two distinct clusters (Asian and America-Europe subgroup) in which our strain (ABT/MVC/CAV2/001) grouped with CAV2 of America-Europe subgroup instead of Asian continent subgroup.This study confirms a novel CAV2 strain using molecular techniques which are genetically distinct in nature from other Indian CAV2 strains that is currently circulating in India.

摘要

2019年至2020年期间，在印度钦奈及其周边地区，从患有肠胃炎和呼吸道感染的犬只身上共采集了26份鼻拭子样本。所有样本均使用通用引物进行PCR，以快速诊断和区分犬腺病毒1型（CAV1）和犬腺病毒2型（CAV2）。只有一个样本产生了1030 bp的扩增子，表明存在CAV2，进一步测序证实了这一点。序列分析显示，与来自巴西、加拿大和美国毒株的其他CAV 2型分离株具有100%的同一性，与其他印度CAV2毒株具有95.9%的同一性。E3基因的系统发育分析显示有两个不同的簇（亚洲和美洲-欧洲亚组），我们的毒株（ABT/MVC/CAV2/001）与美洲-欧洲亚组的CAV2归为一组，而不是亚洲大陆亚组。本研究使用分子技术确认了一种新型CAV2毒株，该毒株在基因上与目前在印度流行的其他印度CAV2毒株不同。