Hogans Madison D, Kretzschmar Will P, Higgins Theresa A, Chakrabortty Atonu, Nance Rebecca L, Smith Bruce F, Bedi Deepa, Sandey Maninder, Agarwal Payal
Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, USA.
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, USA.
Adv Virol. 2022 Dec 22;2022:3658970. doi: 10.1155/2022/3658970. eCollection 2022.
Canine adenovirus type 2 (CAV2) is a nonhuman adenovirus with a known ability to infect human and canine cells. The cell surface receptors involved in CAV2 transduction are still unknown. Identification of these would provide valuable information to develop enhanced gene delivery tools and better understand CAV2 biology. CAV2 is erroneously grouped with Ad5 based on the knowledge that CAV2 may transduce using CAR. Therefore, we have evaluated CAV2 and Ad5 (CAV2GFP, Ad5G/L) infection patterns in various canine and human cell lines to determine their different tropisms. Our research demonstrates that CAV2 can successfully infect cells that Ad5 does not infect, and CAV2 infections do not correlate with CAR expression. CAV2 can infect cells that have a low or minimal expression of CAR. Our data suggest that CAV2 transduction is not dependent on the CAR receptor, and thus, it is crucial to find novel CAV2 receptors.
犬2型腺病毒（CAV2）是一种非人类腺病毒，已知其能够感染人类和犬类细胞。参与CAV2转导的细胞表面受体仍不清楚。鉴定这些受体将为开发增强型基因传递工具和更好地理解CAV2生物学特性提供有价值的信息。基于CAV2可能利用柯萨奇病毒和腺病毒受体（CAR）进行转导这一认识，CAV2被错误地与5型腺病毒（Ad5）归为一类。因此，我们评估了CAV2和Ad5（CAV2GFP、Ad5G/L）在各种犬类和人类细胞系中的感染模式，以确定它们不同的嗜性。我们的研究表明，CAV2能够成功感染Ad5不能感染的细胞，并且CAV2感染与CAR表达无关。CAV2能够感染CAR表达水平低或极低的细胞。我们的数据表明，CAV2转导不依赖于CAR受体，因此，寻找新的CAV2受体至关重要。
