Matsumine Hajime, Niimi Yosuke, Matsumine Hisami
Department of Plastic and Reconstructive Surgery, Toho Women's Clinic, 5-3-10 Kiba, Koto-ku, Tokyo, 135-0042, Japan.
Department of Gynecology, Toho Women's Clinic, 5-3-10 Kiba, Koto-ku, Tokyo, 135-0042, Japan.
Regen Ther. 2021 Apr 16;18:76-81. doi: 10.1016/j.reth.2021.03.008. eCollection 2021 Dec.
Large-animal models such as sheep for facial nerve regeneration research have not yet been established because of the lack of methods for assessing the electrophysiological function of regenerated nerves. In this study, we developed a percutaneous measurement method for the evoked compound muscle action potential (CMAP) of the facial nerve in sheep.
Six 3-year-old castrated male Corriedale sheep were used in this study. Under general anesthesia, an anatomical exploration was performed to identify the course of the buccal branch of the facial nerve and its innervating muscles on one side, followed by the application of surface stimulating electrodes to the contralateral side of the face along the course of the buccal branch of the facial nerve to obtain CMAP measurements of the nasolabial levator muscle.
Percutaneous CMAP measurements of the nasolabial levator muscle could be obtained in all animals by placing stimulating electrodes 1 cm apart on the line coinciding with the course of the buccal branch of the facial nerve revealed by the preceding anatomical exploration. Mean values for electrophysiological parameters were amplitude 4.7 ± 0.7 mV, duration 2.1 ± 0.6 ms, and latency 3.6 ± 0.4 ms.
We have established a percutaneous measurement method for CMAP of the buccal branch of the facial nerve in sheep. This method is expected to be very useful in future studies of facial nerve regeneration for long nerve defects in sheep.
由于缺乏评估再生神经电生理功能的方法，尚未建立用于面神经再生研究的大型动物模型，如绵羊模型。在本研究中，我们开发了一种经皮测量绵羊面神经诱发复合肌肉动作电位（CMAP）的方法。
本研究使用了6只3岁去势雄性考力代绵羊。在全身麻醉下，进行解剖探查以确定一侧面神经颊支的走行及其支配的肌肉，然后将表面刺激电极沿面神经颊支走行应用于对侧面部，以获取鼻唇提肌的CMAP测量值。
通过在前述解剖探查所揭示的与面神经颊支走行一致的线上将刺激电极相距1 cm放置，所有动物均可获得鼻唇提肌的经皮CMAP测量值。电生理参数的平均值为振幅4.7±0.7 mV、持续时间2.1±0.6 ms和潜伏期3.6±0.4 ms。
我们建立了一种经皮测量绵羊面神经颊支CMAP的方法。该方法有望在未来绵羊长神经缺损的面神经再生研究中非常有用。
