Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, Tokyo, Japan.
Department of Physiology, Division of Neurophysiology, Tokyo Women's Medical University, Tokyo, Japan.
J Tissue Eng Regen Med. 2020 Aug;14(8):1087-1099. doi: 10.1002/term.3083. Epub 2020 Jul 9.
In this study, we devised a novel cross-facial nerve grafting (CFNG) procedure using an autologous nerve graft wrapped in an adipose-derived stem cell (ADSC) sheet that was formed on a temperature-responsive dish and examined its therapeutic effect in a rat model of facial palsy. The rat model of facial paralysis was prepared by ligating and transecting the main trunk of the left facial nerve. The sciatic nerve was used for CFNG, connecting the marginal mandibular branch of the left facial nerve and the marginal mandibular branch of the right facial nerve. CFNG alone, CFNG coated with an ADSC suspension, and CFNG wrapped in an ADSC sheet were transplanted in eight rats each, designated the CFNG, suspension, and sheet group, respectively. Nerve regeneration was compared histologically and physiologically. The time to reinnervation, assessed by a facial palsy scoring system, was significantly shorter in the sheet group than in the other two groups. Evoked compound electromyography showed a significantly higher amplitude in the sheet group (4.2 ± 1.3 mV) than in the suspension (1.7 ± 1.2 mV) or CFNG group (1.6 ± 0.8 mV; p < .01). Toluidine blue staining showed that the number of myelinated fibers was significantly higher in the sheet group (2,450 ± 687) than in the suspension (1,645 ± 659) or CFNG group (1,049 ± 307; p < .05). CFNG in combination with ADSC sheets, prepared using temperature-responsive dishes, promoted axonal outgrowth in autologous nerve grafts and reduced the time to reinnervation.
在这项研究中，我们设计了一种新的面-面神经移植（CFNG）方法，使用自体神经移植物包裹在温度响应盘上形成的脂肪干细胞（ADSC）片，并在面瘫大鼠模型中检查其治疗效果。面瘫大鼠模型是通过结扎和切断左侧面神经主干制备的。将坐骨神经用于 CFNG，连接左侧面神经的下颌缘支和右侧面神经的下颌缘支。单独的 CFNG、包裹 ADSC 悬浮液的 CFNG 和包裹 ADSC 片的 CFNG 分别在 8 只大鼠中移植，分别命名为 CFNG、悬浮液和片组。从组织学和生理学上比较神经再生情况。通过面瘫评分系统评估的再神经支配时间，片组明显短于其他两组。诱发复合肌电图显示片组（4.2 ± 1.3 mV）的振幅明显高于悬浮液组（1.7 ± 1.2 mV）或 CFNG 组（1.6 ± 0.8 mV；p <.01）。甲苯胺蓝染色显示片组（2,450 ± 687）的有髓纤维数量明显高于悬浮液组（1,645 ± 659）或 CFNG 组（1,049 ± 307；p <.05）。使用温度响应盘制备的 CFNG 与 ADSC 片联合使用，促进了自体神经移植物中的轴突生长，并缩短了再神经支配的时间。
