Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Cell Mol Med. 2021 Jun;25(12):5823-5827. doi: 10.1111/jcmm.16596. Epub 2021 May 9.
The long non-coding RNAs (lncRNAs) play a critical regulatory role in the host response to the viral infection. However, little is understood about the transcriptome architecture, especially lncRNAs pattern during the SARS-CoV-2 infection. In the present study, using publicly available RNA sequencing data of bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cells (PBMC) samples from COVID-19 patients and healthy individuals, three interesting findings highlighted: (a) More than half of the interactions between lncRNAs-PCGs of BALF samples established by three trans-acting lncRNAs (HOTAIRM1, PVT1 and AL392172.1), which also exhibited the high affinity for binding to the SARS-CoV-2 genome, suggesting the major regulatory role of these lncRNAs during the SARS-CoV-2 infection. (b) lncRNAs of MALAT1 and NEAT1 are possibly contributed to the inflammation development in the SARS-CoV-2 infected cells. (c) In contrast to the 3' part of the SARS-CoV-2 genome, the 5' part can interact with many human lncRNAs. Therefore, the mRNA-based vaccines will not show any side effects because of the off-label interactions with the human lncRNAs. Overall, the putative functionalities of lncRNAs can be promising to design the non-coding RNA-based drugs and to inspect the efficiency of vaccines to overcome the current pandemic.
长链非编码 RNA（lncRNAs）在宿主对病毒感染的反应中发挥着关键的调控作用。然而，人们对其转录组结构，尤其是在 SARS-CoV-2 感染过程中的 lncRNA 模式知之甚少。在本研究中，我们利用公开的 COVID-19 患者和健康个体支气管肺泡灌洗液（BALF）和外周血单核细胞（PBMC）样本的 RNA 测序数据，发现了三个有趣的现象：（a）三种反式作用 lncRNA（HOTAIRM1、PVT1 和 AL392172.1）构建的 BALF 样本中 lncRNA-PCG 之间的相互作用超过一半，这些 lncRNA 与 SARS-CoV-2 基因组也表现出高亲和力，这表明这些 lncRNA 在 SARS-CoV-2 感染过程中发挥着主要的调控作用。（b）lncRNA MALAT1 和 NEAT1 可能有助于 SARS-CoV-2 感染细胞中的炎症发展。（c）与 SARS-CoV-2 基因组的 3' 部分相反，其 5' 部分可以与许多人类 lncRNA 相互作用。因此，mRNA 疫苗不会因为与人类 lncRNA 的非标签相互作用而产生任何副作用。总的来说，lncRNA 的潜在功能可以为设计基于非编码 RNA 的药物以及检查疫苗的功效以克服当前的大流行提供帮助。
