Langer P, Földes O, Gschendtová K
Institute of Experimental Endocrinology, Center of Physiological Sciences SAV, Bratislava, Czechoslovakia.
Horm Metab Res. 1988 Apr;20(4):218-20. doi: 10.1055/s-2007-1010797.
Groups of male rats weighing about 350 g were inserted polyethylene tubings into bile duct and femoral vein under pentobarbital anesthesia. Several iodothyronines (i.e. T4, T3, rT3, 3,5-T2, 3,3'-T2 and 3',5'-T2) were estimated in 2-hr portions of bile with the aid of specific radioimmunoassay. After the infusion of ethanol (0.3 ml/hr/rat for 4 hr) an increase of biliary excretion of rT3 and a decrease of 3,5-T2 was found as compared to controls. When 5 mg linoleic acid was added to 1.2 ml ethanol, the increase of rT3 was significantly higher than that after ethanol only and, in addition, significant increase of 3',5'-T2 excretion was found. It was concluded that both ethanol and unsaturated fatty acids may inhibit 5'-monodeiodination in the liver and that unsaturated nonesterified fatty acids may exert such effect even when administered intravenously without underlying metabolic disorders.
将体重约350克的雄性大鼠分组，在戊巴比妥麻醉下将聚乙烯导管插入胆管和股静脉。借助特异性放射免疫分析法，以2小时为时间段对胆汁中的几种碘甲状腺原氨酸（即T4、T3、反T3、3,5-二碘甲腺原氨酸、3,3'-二碘甲腺原氨酸和3',5'-二碘甲腺原氨酸）进行测定。与对照组相比，在输注乙醇（0.3毫升/小时/只，持续4小时）后，发现反T3的胆汁排泄增加，3,5-二碘甲腺原氨酸减少。当在1.2毫升乙醇中加入5毫克亚油酸时，反T3的增加明显高于仅给予乙醇后的增加，此外，还发现3',5'-二碘甲腺原氨酸排泄显著增加。得出的结论是，乙醇和不饱和脂肪酸都可能抑制肝脏中的5'-单脱碘作用，并且不饱和非酯化脂肪酸即使在没有潜在代谢紊乱的情况下静脉给药也可能发挥这种作用。
