Langer P, Földes O, Straussová K, Gschwendtová K
Endocrinol Exp. 1982 Jun;16(2):117-27.
From a drained bile duct of the rats injected i. v. with various doses of L-thyroxine (50 to 200 microgram per animal) bile was obtained which was then injected to various parts of intestine in recipient rats. The recipient animals were anesthetized with pentobarbiturate and their bile duct as well as femoral artery and vein were cannulated with polyethylene tubings. The level of thyroxine (T4), 3,5,3'-triiodothyronine (T3) and 3,3',5'-triiodothyronine (rT3) in plasma and biliary excretion of these compounds was estimated by specific radioimmunoassay, the samples of plasma and bile being taken up to 4 h after the administration of donor bile. In one experiment also concentration of three different diiodothyronines (e.e. 3,5-T2, 3,3'T2 and 3',5'T2) in recipient bile was measured. A dose dependent increase of T4 excretion was fond in recipient bile. In some experiments also an increase of 3,3'-T2 and 3',5'-T2 excretion was observed and a higher ratio between the excreted and administered amount of rT3 was found than that of T3. An increase of T4 level in plasma was also directly related to the administered dose of this compound, while the increase of T3 and rT3 under the conditions used was observed only in some experiments where relatively higher doses of these substances were administered. It was also observed that the absorption of T4 from the administered bile was higher in fed than in fasted animals.
给大鼠静脉注射不同剂量的L-甲状腺素（每只动物50至200微克），从引流的胆管中获取胆汁，然后将其注射到受体大鼠肠道的各个部位。受体动物用戊巴比妥麻醉，其胆管以及股动脉和静脉用聚乙烯管插管。通过特异性放射免疫测定法估计血浆中甲状腺素（T4）、3,5,3'-三碘甲状腺原氨酸（T3）和3,3',5'-三碘甲状腺原氨酸（rT3）的水平以及这些化合物的胆汁排泄情况，在给予供体胆汁后4小时内采集血浆和胆汁样本。在一项实验中，还测量了受体胆汁中三种不同二碘甲状腺原氨酸（即3,5-T2、3,3'T2和3',5'T2）的浓度。在受体胆汁中发现T4排泄呈剂量依赖性增加。在一些实验中还观察到3,3'-T2和3',5'-T2排泄增加，并且发现rT3排泄量与给药量的比值高于T3。血浆中T4水平的升高也与该化合物的给药剂量直接相关，而在所使用的条件下，仅在一些给予相对较高剂量这些物质的实验中观察到T3和rT3的升高。还观察到，喂食动物从给予的胆汁中吸收T4的量高于禁食动物。
