Kovár J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Hybridoma. 1988 Jun;7(3):255-63. doi: 10.1089/hyb.1988.7.255.
The growth of mouse hybridomas in the defined medium with ferric citrate at the optimum growth-stimulating concentration of 500 microM in place of transferrin is fully comparable with the growth in the medium with transferrin at the optimum concentration of 5 micrograms/ml. Experiments with monoclonal antibody to mouse transferrin receptor demonstrate that the growth-stimulating effect of ferric citrate is not based on the action of residual transferrin or the action of transferrin secreted by hybridoma cells. Such growth-stimulating effect is specific for iron compounds while other metal trace elements are ineffective. Hybridoma cells can absorb iron from medium containing iron salt without the presence of transferrin.
在含有柠檬酸铁的限定培养基中，以最佳生长刺激浓度500微摩尔代替转铁蛋白培养小鼠杂交瘤，其生长情况与在含有最佳浓度5微克/毫升转铁蛋白的培养基中生长完全相当。用抗小鼠转铁蛋白受体单克隆抗体进行的实验表明，柠檬酸铁的生长刺激作用并非基于残留转铁蛋白的作用或杂交瘤细胞分泌的转铁蛋白的作用。这种生长刺激作用对铁化合物具有特异性，而其他金属微量元素则无效。杂交瘤细胞可以在不存在转铁蛋白的情况下从含铁盐的培养基中吸收铁。
