体外评估基于三羟甲基氨基甲烷卵黄和大豆卵磷脂的稀释液在杂交公羊精液冷冻保存中的应用。

In vitro Assessment of Tris Egg Yolk and Soybean Lecithin Based Extenders for Cryopreservation of Crossbred Ram Semen.

机构信息

Division of Animal Reproduction, Gynaecology and Obstetrics, Faculty of Veterinary Sciences and Animal Husbandry, Shere-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Shuhama, Alusteng, Jammu and Kashmir, India.

Division of Veterinary Clinical Complex, Faculty of Veterinary Sciences and Animal Husbandry, Shere-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Shuhama, Alusteng, Jammu and Kashmir, India.

出版信息

Cryo Letters. 2021 Mar-Apr;42(2):73-80.

PMID:33970983

Abstract

BACKGROUND

The replacement of egg yolk with alternative plant-derived soybean lecithin is gaining interest in both animal and human sperm cryopreservation owing to biosecurity issues with egg yolk based extenders.

OBJECTIVE

To evaluate the comparative effect of egg yolk and soyabean lecithin based extenders on the quality of cryopreserved crossbred ram semen.

METHODS

Pooled ejaculates (total ejaculates = 36) were divided into two aliquots and extended with Tris egg yolk extender (Tris extender) and soybean lecithin based commercial extender (Ovixcell) RESULTS: Among the two extenders, Ovixcell showed better sperm quality both at the pre-freeze (Sperm motility) and post-thaw stages. Lower malondialdehyde (MDA) level (nmol/mL) was observed in Ovixcell as compared to Tris extender. Both sperm quality and MDA level decreased significantly (P < 0.05) from pre-freeze to post-thaw in both the extenders.

CONCLUSION

The findings of the present study indicate that Ovixcell is a comparable alternative to Tris extender for the cryopreservation of crossbred ram semen.

摘要

背景

由于蛋黄基 extender 存在生物安全问题，用替代植物源性大豆卵磷脂替代蛋黄在动物和人类精子冷冻保存中引起了关注。

目的

评估蛋黄和大豆卵磷脂基 extender 对冷冻杂交公羊精液质量的比较效果。

方法

将混合精液（总精液量=36）分为两等份，分别用 Tris 蛋黄 extender（Tris extender）和大豆卵磷脂商业 extender（Ovixcell）进行扩展。

结果

在两种 extender 中，Ovixcell 在冷冻前（精子活力）和冷冻后阶段均显示出更好的精子质量。与 Tris extender 相比，Ovixcell 中的丙二醛（MDA）水平（nmol/mL）较低。两种 extender 的精子质量和 MDA 水平在冷冻前和冷冻后均显著降低（P < 0.05）。