Division of Animal Reproduction, Gynaecology and Obstetrics, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar-190006 UT Jammu and Kashmir, India.
Krishi Vigyan Kendra, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Kargil-1, 194103, UT Ladakh, India.
Cryo Letters. 2021 May-Jun;42(3):146-153.
Antioxidants reduce oxidative stress and improve sperm quality during cryopreservation.
To investigate the effect of idebenone, resveratrol and taurine on the sperm quality and lipid peroxidation of cryopreserved crossbred ram semen.
In a split study, pooled ejaculates were divided into four aliquots cryopreserved in tris extender with no antioxidant (control), with idebenone (0.01 mM), resveratrol (0.1 mM), and taurine (40 mM).
Among all antioxidants, taurine treatment yielded significantly better sperm quality. Malondialdehyde (MDA) level in seminal plasma was significantly lower for taurine compared to the control, idebenone and resveratrol treatments. Moreover, sperm quality declined significantly in all the groups from pre-freeze to post-thaw.
The findings indicate that taurine at 40 mM significantly improves sperm quality compared to 0.01 mM idebenone and 0.1 mM resveratrol, hence it can be considered as a potent and promising antioxidant supplement in tris extender for the cryopreservation of crossbred ram semen.
抗氧化剂可减少冷冻保存过程中的氧化应激并改善精子质量。
研究依地酸二钠、白藜芦醇和牛磺酸对冷冻保存杂交公绵羊精液质量和脂质过氧化的影响。
在一项分割研究中，将合并的精液分为四等份，分别在不含抗氧化剂的 tris 稀释液（对照）、含 0.01mM 依地酸二钠、0.1mM 白藜芦醇和 40mM 牛磺酸的 tris 稀释液中冷冻保存。
在所有抗氧化剂中，牛磺酸处理的精子质量显著更好。与对照组、依地酸二钠和白藜芦醇处理组相比，牛磺酸处理组的精浆丙二醛（MDA）水平显著降低。此外，所有组的精子质量在冷冻前和冷冻后都显著下降。
研究结果表明，与 0.01mM 依地酸二钠和 0.1mM 白藜芦醇相比，40mM 牛磺酸可显著提高精子质量，因此，它可以被认为是 tris 稀释液中冷冻保存杂交公绵羊精液的一种有效且有前途的抗氧化补充剂。
