Jiang Jingwei, Zhao Zelong, Gao Shan, Chen Zhong, Dong Ying, He Ping, Wang Bai, Pan Yongjia, Wang Xuda, Guan Xiaoyan, Wang Chao, Lin Shanshan, Sun Hongjuan, Zhou Zunchun
Liaoning Key Lab of Germplasm Improvement and Fine Seed Breeding of Marine Aquatic Animals, Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
Liaoning Key Lab of Germplasm Improvement and Fine Seed Breeding of Marine Aquatic Animals, Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
Comp Biochem Physiol Part D Genomics Proteomics. 2021 Sep;39:100845. doi: 10.1016/j.cbd.2021.100845. Epub 2021 May 6.
The sea cucumber Apostichopus japonicus is an economically important marine organism, and its aquaculture has rapidly developed in China. The very large market demand puts forward higher requirements for the economically efficient breeding of sea cucumbers. Sex and the associated reproductive processes have been reported to affect the physiological characteristics of sea cucumbers. However, little is known about the metabolism differences that related to sex and the associated reproductive processes and their potential effects on the efficiency of A. japonicus aquaculture. In this study, ultra-performance liquid chromatography was applied to investigate the variations in metabolic profiles in cell-free coelomic fluids (CCFs) of sea cucumbers of different sexes and reproductive states. A total of 4435 metabolites were detected, and the metabolic profiles of A. japonicus were significantly affected by both sexes and reproductive process. The differentially abundant metabolites in CCFs of A. japonicus of different sexes and reproductive states were also screened and analyzed. The findings revealed that unsaturated fatty acid synthesis and phenylalanine metabolism were the most significantly changed pathways. Moreover, the weakest ability to synthesize capsaicin using phenylalanine was found in A. japonicus after spawning. Our study provides new insights into the metabolic response of A. japonicus during the reproductive process, and also provides valuable references for the economically efficient breeding of A. japonicus.
刺参是一种具有重要经济价值的海洋生物，其在中国的水产养殖发展迅速。巨大的市场需求对刺参的高效养殖提出了更高要求。据报道，性别及相关生殖过程会影响刺参的生理特征。然而，关于与性别及相关生殖过程有关的代谢差异及其对刺参养殖效率的潜在影响，人们了解甚少。在本研究中，采用超高效液相色谱法研究不同性别和生殖状态的刺参无细胞体腔液（CCF）中代谢谱的变化。共检测到4435种代谢物，刺参的代谢谱受性别和生殖过程的显著影响。还对不同性别和生殖状态的刺参CCF中差异丰富的代谢物进行了筛选和分析。研究结果表明，不饱和脂肪酸合成和苯丙氨酸代谢是变化最显著的途径。此外，产卵后的刺参利用苯丙氨酸合成辣椒素的能力最弱。我们的研究为刺参生殖过程中的代谢反应提供了新的见解，也为刺参的高效养殖提供了有价值的参考。
