CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China; CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China; CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Food Chem. 2021 Aug 1;352:129339. doi: 10.1016/j.foodchem.2021.129339. Epub 2021 Feb 25.
The sea cucumber, Apostichopus japonicas, is an important economic species with high nutritive value. In recent years, driven by significant market demand, the sea cucumber breeding industry has developed rapidly. Body color and number of papillae are important factors that determine the value and price of sea cucumbers. In this study, metabolite profiling of four sea cucumber varieties (green, white, purple and spiny) was performed using ultra-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-QTOF-MS) combined with multivariate analysis. Orthogonal partial least-squares discriminant analysis (OPLS-DA) clearly discriminated the body wall metabolites of four sea cucumber varieties. Differential metabolites included fatty acids, phospholipids, and sugars. KEGG metabolic pathway analysis revealed that carbohydrate, protein, and phospholipid metabolism were highly conserved among the varieties. These results provide a comprehensive insight into differences in the metabolite profile of four A. japonicus varieties and a deeper understanding of sea cucumber varieties breeding.
海参，刺参，是一种具有高营养价值的重要经济物种。近年来，由于市场需求巨大，海参养殖业发展迅速。体色和乳突数是决定海参价值和价格的重要因素。在这项研究中，我们采用超高效液相色谱-四极杆飞行时间质谱联用（UPLC-QTOF-MS）结合多元分析方法对四种海参（绿色、白色、紫色和刺参）的代谢物进行了分析。正交偏最小二乘判别分析（OPLS-DA）清楚地区分了四种海参的体壁代谢物。差异代谢物包括脂肪酸、磷脂和糖。KEGG 代谢途径分析表明，不同品种之间的碳水化合物、蛋白质和磷脂代谢高度保守。这些结果提供了对四种刺参品种代谢组图谱差异的全面了解，也加深了对海参品种选育的认识。
