中华绒螯蟹外套膜小核糖核蛋白参与贝壳和珍珠层颜色形成。
The mantle exosome proteins of Hyriopsis cumingii participate in shell and nacre color formation.
机构信息
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai 201306, China; Fisher Institute of Anhui Academy of Agricultural Sciences, Hefei 230031, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China.
出版信息
Comp Biochem Physiol Part D Genomics Proteomics. 2021 Sep;39:100844. doi: 10.1016/j.cbd.2021.100844. Epub 2021 May 1.
Pearl color is closely related to the nacre color of shell in Hyriopsis cumingii, and pearl color has a huge impact on its price. The nacre is an important part of the shell, and studies have suggested that mantle exosomes participated in shell formation. Exosomes contain many different proteins that are involved in different biological processes. In this study, exosomes were extracted from mantles of mussels with different nacre color. TMT quantitative proteome sequencing analysis was performed on purple and white mussel mantle exosomes, and 4861 proteins were obtained. Based on the standard of (|log2 (Fold change)| ≥ 1.2 or ≤ 0.83 and p-value ≤ 0.05), a total of 758 differentially expressed proteins were found. Some proteins involved in shell and nacre color formation were predicted with the proteins annotate, GO classification system. Moreover, 14 differentially expressed proteins (including eight up-regulated proteins and six down-regulated proteins) were validated using parallel reaction monitoring (PRM) assays. Overall, this information will be useful to improve our understanding of the molecular mechanisms of shell and nacre color formation in H. cumingii.
珍珠的颜色与三角帆蚌贝壳的珍珠层颜色密切相关，而珍珠的颜色对其价格有巨大影响。珍珠层是贝壳的重要组成部分，研究表明，套膜外泌体参与了贝壳的形成。外泌体中含有许多不同的蛋白质，这些蛋白质参与了不同的生物过程。在这项研究中，从具有不同珍珠层颜色的贻贝套膜中提取了外泌体。对紫色和白色贻贝套膜外泌体进行了 TMT 定量蛋白质组学测序分析，共获得 4861 种蛋白质。基于（|log2（倍数变化）|≥1.2 或≤0.83 和 p 值≤0.05）的标准，共发现了 758 种差异表达蛋白。利用蛋白质注释、GO 分类系统预测了一些与贝壳和珍珠层颜色形成有关的蛋白质。此外，使用平行反应监测（PRM）测定法验证了 14 种差异表达蛋白（包括 8 种上调蛋白和 6 种下调蛋白）。总的来说，这些信息将有助于提高我们对三角帆蚌贝壳和珍珠层颜色形成的分子机制的理解。
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