miR-4504 参与了三角帆蚌珍珠层颜色的形成。
miR-4504 is involved in nacre color formation in Hyriopsis cumingii.
机构信息
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China.
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
出版信息
Biochem Biophys Res Commun. 2019 Sep 17;517(2):210-215. doi: 10.1016/j.bbrc.2019.07.054. Epub 2019 Jul 20.
Pearl color is affected by the nacre color of shells in Hyriopsis cumingii, and is the primary indicator of its value. MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play important roles in many biological processes, including pigmentation. In this study, we used a luciferase reporter assay to identify that miR-4504 can interact with the 3'-untranslated region of the MITF gene in H. cumingii (HcMitf). After injecting mussels with the miR-4504 antagomir, the expression of miR-4504 was inhibited. Upon miR-4505 silencing, the expression of HcMitf and its downstream gene, HcTyr, were simultaneously increased. Tyrosinase activity and melanin content were also increased. The collective findings indicated that miR-4504 was involved in melanin synthesis in H. cumingii. These findings also improve our understanding of the molecular mechanisms of nacre color formation in H. cumingii.
珍珠的颜色受三角帆蚌贝壳中珍珠层颜色的影响，是其价值的主要指标。微小 RNA（miRNA）是内源性的小非编码 RNA，在包括色素沉着在内的许多生物过程中发挥重要作用。在这项研究中，我们使用荧光素酶报告基因 assay 鉴定出 miR-4504 可以与三角帆蚌（HcMitf）MITF 基因的 3'非翻译区相互作用。向贻贝注射 miR-4504 反义寡核苷酸后，miR-4504 的表达受到抑制。miR-4505 沉默后，HcMitf 及其下游基因 HcTyr 的表达同时增加。酪氨酸酶活性和黑色素含量也增加。研究结果表明，miR-4504 参与了三角帆蚌黑色素的合成。这些发现也提高了我们对三角帆蚌珍珠层颜色形成分子机制的理解。
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