Mestria Serena, Odoardi Sara, Biosa Giulia, Valentini Valeria, Di Masi Gerardo, Cittadini Francesca, Strano-Rossi Sabina
Department of Healthcare Surveillance and Bioethics, Section of Legal Medicine, Università Cattolica del Sacro Cuore F. Policlinico Gemelli IRCCS, Rome, Italy.
Department of Healthcare Surveillance and Bioethics, Section of Legal Medicine, Università Cattolica del Sacro Cuore F. Policlinico Gemelli IRCCS, Rome, Italy.
Forensic Sci Int. 2021 May 4;323:110817. doi: 10.1016/j.forsciint.2021.110817.
The constant increase of new psychoactive substances, often available on the illicit drug market as 'research chemicals', poses a concern for public health and a significant analytical and legislative challenge. Β-keto-arylcyclohexamines represent a class of dissociative anesthetics recently introduced on the market of New Psychoactive Substances (NPS). There is still a lack of information about the pharmacological activity of many of such substances, usually depending on the potential chemical modifications introduced to circumvent the law. Furthermore, their intake may not be fully intentional, since consumers do not always have knowledge of the content of online purchases. The present study describes the characterization by liquid chromatography-high resolution mass spectrometry (LC-HRMS), using a benchtop Orbitrap instrument, of the novel ketamine analogues methoxpropamine, 2-fluoro-deschloroketamine and deschloroketamine, found in the post-mortem blood and hair samples from a forensic case of suicide by fall from height, and of some of their metabolites. This allowed the development of analytical methods for the determination of both the β-keto-arylcyclohexamines and the metabolites in LC-HRMS and in LC-MS/MS, providing a starting point for studying their toxicokinetics.
新型精神活性物质不断增加，常在非法毒品市场上作为“科研化学品”出现，这对公众健康构成了担忧，也是一项重大的分析和立法挑战。β-酮芳基环己胺是一类最近出现在新型精神活性物质（NPS）市场上的分离麻醉剂。关于许多此类物质的药理活性仍然缺乏信息，这通常取决于为规避法律而进行的潜在化学修饰。此外，它们的摄入可能并非完全出于故意，因为消费者并不总是了解网上购买物品的成分。本研究描述了使用台式轨道阱仪器通过液相色谱-高分辨率质谱（LC-HRMS）对新型氯胺酮类似物甲氧丙胺、2-氟去氯胺酮和去氯胺酮进行的表征，这些物质是在一例高处坠落自杀法医案件的死后血液和毛发样本中发现的，还包括它们的一些代谢物。这使得能够开发用于在LC-HRMS和LC-MS/MS中测定β-酮芳基环己胺及其代谢物的分析方法，为研究它们的毒代动力学提供了一个起点。
