Nanjing Institute for Comprehensive Utilization of Wild Plants, Nanjing, 211100, China.
Shandong Jiuxin Biological Technology Co., Ltd., Shandong, 271500, China.
Mol Immunol. 2021 Jul;135:312-319. doi: 10.1016/j.molimm.2021.04.019. Epub 2021 May 7.
Candida albicans is an opportunistic fungal human pathogen that has been causing an increasing number of deaths each year. Due to the widespread use of broad-spectrum antibiotics and immunosuppressants, C. albicans resistance to these therapies has increased. Thus, natural plant inhibitors are being investigated for treating C. albicans infections. Schinifoline is a 4-quinolinone alkaloid with antibacterial, insecticidal, antitumor, and other biological activities. Here, we explored the effects of schinifoline on C. albicans in C. elegans and extracted RNA from uninfected C. elegans, C. elegans infected with C. albicans, and C. elegans infected with C. albicans and treated with 100 mg/l schinifoline. Our results showed that there were significant differences among the three groups. The GO and KEGG pathway analysis suggested that the pathogenicity of C. albicans to C. elegans was caused by abnormal protein function. Schinifoline regulates lysosomal pathway related genes that accelerate the metabolism and degradation of abnormal proteins, thereby inhibiting the negative effects of C. albicans in vivo. These findings advance our understanding of the molecular mechanisms underlying schinifoline inhibition of C. albicans.
白色念珠菌是一种机会性真菌病原体，每年导致的死亡人数不断增加。由于广谱抗生素和免疫抑制剂的广泛使用，白色念珠菌对这些治疗方法的耐药性增加。因此，正在研究天然植物抑制剂来治疗白色念珠菌感染。蛇菰宁是一种具有抗菌、杀虫、抗肿瘤等生物活性的 4-喹啉酮生物碱。在这里，我们研究了蛇菰宁对秀丽隐杆线虫中白色念珠菌的影响，并从未感染的秀丽隐杆线虫、感染白色念珠菌的秀丽隐杆线虫和用 100mg/L 蛇菰宁处理的感染白色念珠菌的秀丽隐杆线虫中提取 RNA。我们的结果表明，这三组之间存在显著差异。GO 和 KEGG 通路分析表明，白色念珠菌对秀丽隐杆线虫的致病性是由异常蛋白功能引起的。蛇菰宁调节溶酶体途径相关基因，加速异常蛋白的代谢和降解，从而抑制白色念珠菌在体内的负面影响。这些发现增进了我们对蛇菰宁抑制白色念珠菌的分子机制的理解。
