Department of Zoology, Mizoram University Aizawl, 796 004, India.
Burns. 2022 Feb;48(1):132-145. doi: 10.1016/j.burns.2021.04.016. Epub 2021 Apr 21.
Wound injury is common and causes serious complications if not treated properly. The moist dressing heals wounds faster than other dressings. Therefore, we sought to study the effect of hesperidin/naringin hydrogel wound dressing or their combinations on the deep dermal wounds in mice.
A rectangular full thickness skin flap of 2.5 × 1.5 cm was excised from depilated mice dorsum and the wound was fully covered with 5% hesperidin/5% naringin hydrogel or both in the ratio of 1:1, 2:1, or 1:2, respectively once daily until complete healing of the wound. Data were collected on wound contraction, mean wound healing time, collagen, DNA, and nitric oxide syntheses, glutathione concentration, superoxide dismutase activity, and lipid peroxidation throughout healing. Expression of NF-κB and COX-2 were also estimated in the regenerating granulation tissue using Western blot.
Dressing of wounds with 5% hesperidin hydrogel led to a higher and early wound contraction and significantly reduced mean wound healing time by 5.7 days than 5% naringin or combination of hesperidin and naringin hydrogels in the ratio of 1:1, 2:1, or 1:2. Hesperidin hydrogel wound dressing caused higher collagen and DNA syntheses than other groups at all times after injury. Glutathione concentration and superoxide dismutase activity increased followed by a decline in lipid peroxidation in regenerating wounds after hesperidin/naringin hydrogel application and a maximum effect was observed for hesperidin alone. The hesperidin/naringin hydrogel suppressed NF-κB and COX-2 expression on days 6 and 12.
Application of 5% hesperidin hydrogel was more effective than 5% naringin or combination of hesperidin and naringin gels (1:1, 2:1 or 1:2) indicated by a greater wound contraction, reduced mean wound healing time, elevated collagen and DNA syntheses, rise in glutathione concentration, and superoxide dismutase activity followed by reduced lipid peroxidation, and NF-κB, and COX-2 expression.
伤口损伤很常见，如果处理不当会导致严重的并发症。湿性敷料比其他敷料更快地治愈伤口。因此，我们试图研究橙皮苷/柚皮苷水凝胶敷料或它们的组合对小鼠真皮深层伤口的影响。
从去毛的小鼠背部切除 2.5×1.5cm 的矩形全厚皮瓣，用 5%橙皮苷/5%柚皮苷水凝胶或两者以 1:1、2:1 或 1:2 的比例分别每天一次完全覆盖伤口，直至伤口完全愈合。收集伤口收缩、平均愈合时间、胶原、DNA 和一氧化氮合成、谷胱甘肽浓度、超氧化物歧化酶活性和脂质过氧化的数据。使用 Western blot 还估计了再生肉芽组织中 NF-κB 和 COX-2 的表达。
用 5%橙皮苷水凝胶处理伤口导致更高和更早的伤口收缩，并比 5%柚皮苷或橙皮苷/柚皮苷水凝胶以 1:1、2:1 或 1:2 的比例的组合显著减少平均愈合时间 5.7 天。橙皮苷水凝胶敷料在受伤后所有时间都导致更高的胶原和 DNA 合成，比其他组都高。在橙皮苷/柚皮苷水凝胶应用后再生伤口中，谷胱甘肽浓度和超氧化物歧化酶活性增加，随后脂质过氧化减少，橙皮苷单独应用时效果最大。橙皮苷/柚皮苷水凝胶抑制了 NF-κB 和 COX-2 的表达在第 6 天和第 12 天。
与 5%柚皮苷或橙皮苷/柚皮苷水凝胶（1:1、2:1 或 1:2）组合相比，应用 5%橙皮苷水凝胶的效果更好，表现为更大的伤口收缩、减少的平均愈合时间、升高的胶原和 DNA 合成、谷胱甘肽浓度和超氧化物歧化酶活性升高，随后脂质过氧化、NF-κB 和 COX-2 表达减少。
