产 KPC-2 型碳青霉烯酶的肺炎克雷伯菌临床分离株中，一种对头孢他啶-阿维巴坦耐药的 KPC-82 型碳青霉烯酶的特性研究。
Characterization of KPC-82, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam in a Carbapenem-Nonsusceptible Clinical Isolate of Citrobacter koseri.
机构信息
Multidrug-Resistant Organism Repository and Surveillance Network (MRSN), Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
出版信息
Antimicrob Agents Chemother. 2021 Jun 17;65(7):e0015021. doi: 10.1128/AAC.00150-21.
KPC-82 is a KPC-2 variant identified in a carbapenem-nonsusceptible Citrobacter koseri that confers high-level resistance to ceftazidime-avibactam. Genomic analysis revealed that is carried by a chromosomally integrated Tn transposon (disrupting porin gene ) and evolved by a 6-nucleotide tandem repeat duplication causing a two-amino-acid insertion (Ser-Asp) within the Ala-Ser loop. Similar to related KPC variants, KPC-82 showed decreased carbapenemase activity when expressed in a heterologous background and remained susceptible to carbapenem/β-lactamase inhibitor combinations.
KPC-82 是一种在对碳青霉烯类药物不敏感的柠檬酸杆菌中发现的 KPC-2 变体，它对头孢他啶-阿维巴坦具有高度耐药性。基因组分析显示， 由染色体整合的 Tn 转座子携带（破坏孔蛋白基因 ），并通过 6 个核苷酸串联重复导致 Ala-Ser 环内的两个氨基酸插入（Ser-Asp）而进化而来。与相关的 KPC 变体类似，KPC-82 在异源背景中表达时显示出降低的碳青霉烯酶活性，并且仍然对碳青霉烯类/β-内酰胺酶抑制剂组合敏感。
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