Almadori G, Bastianini L, Bistoni F, Paludetti G, Rosignoli M
Department of Otorhinolaryngology, Catholic University of the Sacred Heart, Rome, Italy.
Int J Pediatr Otorhinolaryngol. 1988 May;15(2):157-62. doi: 10.1016/0165-5876(88)90067-5.
Surface and 'core' tonsillar specimens were collected from 60 children, between 2 and 14 years of age, suffering from recurrent tonsillitis, in order to establish the reliability of surface tonsillar swabbing technique and to define the most frequently involved aerobic and anaerobic microorganisms. From the qualitative point of view, the same mixed aerobic and anaerobic flora were obtained in both samples thus demonstrating the reliability of the surface swabbing technique. From the quantitative point of view, 187 microorganisms, 129 (69.2%) aerobes and 58 (30.8%) anaerobes were isolated from surface cultures while 184, 109 (59.2%) aerobes and 75 (40.8%) anaerobes from the core cultures, thus demonstrating a greater number of anaerobes in the core of the tonsil. The most common isolated aerobic microorganisms were the alpha- and beta-hemolytic Streptococci, the Neisseriae and the Staphylococcus aureus; the predominant anaerobic ones were the Fusobacterium nucleatum, the Bacteroides sp. and the Veillonella parvula. The isolated staphylococcus aureus and the bacteroides sp. were all beta-lactamase producers.
从60名2至14岁患有复发性扁桃体炎的儿童身上采集了扁桃体表面和“核心”样本，以确定扁桃体表面拭子技术的可靠性，并确定最常涉及的需氧和厌氧微生物。从定性的角度来看，两个样本中均获得了相同的需氧和厌氧混合菌群，从而证明了表面拭子技术的可靠性。从定量的角度来看，从表面培养物中分离出187种微生物，其中129种（69.2%）为需氧菌，58种（30.8%）为厌氧菌；而从核心培养物中分离出184种，其中109种（59.2%）为需氧菌，75种（40.8%）为厌氧菌，从而表明扁桃体核心中的厌氧菌数量更多。最常见的分离需氧微生物是α-和β-溶血性链球菌、奈瑟菌属和金黄色葡萄球菌；主要的厌氧微生物是具核梭杆菌、拟杆菌属和小韦荣球菌。分离出的金黄色葡萄球菌和拟杆菌属均为β-内酰胺酶产生菌。
