Guo Hao, Zhang Linlei
Department of General Surgery, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
Biochem Genet. 2021 Dec;59(6):1544-1557. doi: 10.1007/s10528-021-10075-6. Epub 2021 May 10.
Early growth response (EGR) proteins have been reported to be involved in cell growth and apoptosis in a variety of cancer types and could inhibit tumor development. However, the role of EGR1/2 in papillary thyroid carcinoma (PTC) has not been elucidated. The expression pattern of EGR1/2 in adjacent tissues and cancer tissues and the clinical prognosis of EGR1/2 were analyzed by using the samples from TCGA database. The cell viability was detected by MTT assay. Luciferase reporter assay was used to demonstrate the binding of EGR1/2 to the target gene promotor region. Our results showed that EGR1/2 was significantly downregulated in tumor tissues and correlated with poor prognosis. Overexpression of EGR1/2 inhibited proliferation of IHH-4 and BCPAP cells, and knockdown of EGR1/2 showed a reverse effect. Overexpression of EGR1 or EGR2 promoted phosphatase and tension homolog (PTEN) or Bcl-2-associated X (BAX) expression, and EGR1 or EGR2 was able to directly bind to the promoter region of PTEN or BAX. In conclusion, we found that the altered expression of EGR1/2 affected the proliferation of PTC cells and regulated the expression of PTEN and BAX.
据报道，早期生长反应（EGR）蛋白参与多种癌症类型的细胞生长和凋亡，并可抑制肿瘤发展。然而，EGR1/2在甲状腺乳头状癌（PTC）中的作用尚未阐明。利用TCGA数据库的样本分析了EGR1/2在癌旁组织和癌组织中的表达模式以及EGR1/2的临床预后。通过MTT法检测细胞活力。荧光素酶报告基因检测用于证明EGR1/2与靶基因启动子区域的结合。我们的结果表明，EGR1/2在肿瘤组织中显著下调，且与预后不良相关。EGR1/2的过表达抑制了IHH-4和BCPAP细胞的增殖，而敲低EGR1/2则显示出相反的效果。EGR1或EGR2的过表达促进了磷酸酶和张力蛋白同源物（PTEN）或Bcl-2相关X蛋白（BAX）的表达，并且EGR1或EGR2能够直接结合到PTEN或BAX的启动子区域。总之，我们发现EGR1/2表达改变影响了PTC细胞的增殖，并调节了PTEN和BAX的表达。
