State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
Collaborative Innovation Center for Improving Quality and Increase Profits of Protected Vegetables in Shanxi, Shanxi Agricultural University, Taigu, China.
Mol Biotechnol. 2021 Jul;63(7):638-649. doi: 10.1007/s12033-021-00332-9. Epub 2021 May 10.
Carotenoids are the general term of natural pigments. The formation of plant color is probably related to the components of carotenoids. As the yellow variety of celery, it is rich in the composition and content of carotenoids. However, the transcript profiling and roles of the genes related to carotenoids biosynthesis in yellow celery remain unclear. In this study, three yellow celery cultivars at different growth stages were used to analyze the content and composition of carotenoids and transcriptional changes of carotenoid biosynthesis-related genes. The lutein and β-carotene were detected in yellow celery cultivar, while α-carotene and lycopene were not detected. The contents of lutein and β-carotene were higher in leaf blades than in petioles. During the growth and development, the contents of lutein and β-carotene gradually decreased in celery. Compared with the other two cultivars, the contents of lutein and β-carotene were the highest in 'Huangtaiji' of 65 days after sowing (DAS) and 85 DAS and 'Liuhehuangxinqin' of 105 DAS, respectively. The expression levels of AgLCYB and AgPSY2 genes were significantly correlated with lutein and β-carotene contents. This work provided a reference for the further study on carotenoid metabolisms in yellow celery and also made sense on the way of cultivating yellow celery with high carotenoids content.
类胡萝卜素是天然色素的统称。植物颜色的形成可能与类胡萝卜素的成分有关。作为芹菜的黄色品种，它富含类胡萝卜素的组成和含量。然而，黄色芹菜中与类胡萝卜素生物合成相关的基因的转录谱和作用仍不清楚。本研究以 3 个不同生长阶段的黄色芹菜品种为试材，分析了类胡萝卜素的含量和组成，以及类胡萝卜素生物合成相关基因的转录变化。在黄色芹菜品种中检测到叶黄素和β-胡萝卜素，而未检测到α-胡萝卜素和番茄红素。叶黄素和β-胡萝卜素在叶片中的含量高于叶柄。在生长发育过程中，芹菜中叶黄素和β-胡萝卜素的含量逐渐降低。与其他两个品种相比，‘黄台吉’在播种后 65 天（DAS）和 85 DAS 以及‘六河黄心芹’在 105 DAS 时叶黄素和β-胡萝卜素的含量最高。AgLCYB 和 AgPSY2 基因的表达水平与叶黄素和β-胡萝卜素含量呈显著正相关。本研究为进一步研究黄色芹菜中的类胡萝卜素代谢提供了参考，也为培育高类胡萝卜素含量的黄色芹菜提供了思路。
