Chen Fan, He Jian'an, Dong Ruiling, Yang Fan, Liu Houming, Gu Dayong, Wang Wei
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Science, Hubei University, Wuhan 430062, Hubei, China.
Shenzhen International Travel Health Care Center, Shenzhen 518033, Guangdong, China.
Sheng Wu Gong Cheng Xue Bao. 2021 Apr 25;37(4):1360-1367. doi: 10.13345/j.cjb.200497.
Imported malaria has become a major risk factor for malaria prevention and control in China. How to screen malaria quickly for people entering China is an urgent problem to be solved. Protein microarrays are widely used in high-throughput screening and diagnosis. In this study, surface plasmon resonance (SPR) technique for malaria detection was established by using the specific adsorption surface treated by polyethylene glycol polymer, and the malaria specific antigen HRP2 was used as capture probe. The optimal concentration of antigen, sensitivity and specificity of detection, as well as anti-interference ability of the chip were analyzed. The SPR protein chip was applied to detect specific antibodies of malignant malaria in serum with the advantage of label-free, instant and fast. Compared with fluorescence quantitative PCR, there were no significant difference in sensitivity and specificity between the two methods. This study lays a foundation for further development of protein microarray for malaria typing identification, and it is conducive to the rapid screening of malaria for people entering.
输入性疟疾已成为我国疟疾防控的主要危险因素。如何对入境人员快速筛查疟疾是亟待解决的问题。蛋白质芯片广泛应用于高通量筛选与诊断。本研究利用聚乙二醇聚合物处理的特异性吸附表面建立了用于疟疾检测的表面等离子体共振（SPR）技术，以疟疾特异性抗原HRP2作为捕获探针。分析了抗原最佳浓度、检测的灵敏度和特异性以及芯片的抗干扰能力。SPR蛋白质芯片应用于血清中恶性疟原虫特异性抗体的检测，具有无需标记、即时、快速的优点。与荧光定量PCR相比，两种方法在灵敏度和特异性上无显著差异。本研究为进一步研发用于疟疾分型鉴定的蛋白质芯片奠定了基础，有利于对入境人员进行疟疾快速筛查。
