Nyberg L, Lundström K, Edfors-Lilja I, Rundgren M
Dept. of Clin. Chem., College of Vet. Med., Swedish University of Agricultural Sciences, Uppsala.
J Anim Sci. 1988 May;66(5):1201-11. doi: 10.2527/jas1988.6651201x.
Effects of stress on concentrations of cortisol and corticosteroid-binding globulin (CBG) in blood plasma and on glucocorticoid receptor concentrations in muscle cytosol were studied in pigs representing three Halothane (Hal) genotypes (NN, Nn, nn). At 12 wk of age, animals were divided into four groups: pigs subjected to transport (5 h in a truck), pigs treated with amperozide prior to transport, pigs not transported but treated with amperozide and pigs neither transported nor given amperozide. Animals were slaughtered the week they reached 100 kg live weight (3 mo later). The Hal gene showed no major influence on the variables studied except for cortisol concentrations (P = .06) measured directly after transport at 12 wk of age (NN = 66.8 nM, Nn = 61.4 nM, nn = 69.4 nM). However, the response in each Hal genotype differed, depending on whether or not the pigs had been exposed to transport. Two weeks after transport, NN pigs developed higher cortisol concentrations than untransported animals, whereas the response was reversed in nn animals; Nn pigs showed no difference in this regard. At slaughter, the effect of transport (12 wk of age) on cortisol and CBG was still apparent. In NN pigs cortisol and CBG concentrations were elevated (P less than or equal to .05, P = .08, respectively), whereas concentrations tended to be lower in nn pigs (P = .17, P = .07, respectively) when compared with untransported pigs. Transported pigs had lower receptor concentrations at slaughter (P less than or equal to .01) than untransported pigs. However, pigs given amperozide in connection with transport had a receptor concentration comparable to that in untransported pigs. Our study shows conclusively that transport stress had long-term effects on cortisol, CBG and glucocorticoid receptor concentrations. In addition, amperozide had long-term effects on cortisol and receptor concentrations.
研究了应激对代表三种氟烷（Hal）基因型（NN、Nn、nn）的猪血浆中皮质醇和皮质类固醇结合球蛋白（CBG）浓度以及肌肉细胞溶胶中糖皮质激素受体浓度的影响。在12周龄时，将动物分为四组：运输的猪（在卡车中5小时）、运输前用安哌齐特处理的猪、未运输但用安哌齐特处理的猪以及既未运输也未给予安哌齐特的猪。动物在达到100千克活重时（3个月后）被屠宰。除了12周龄运输后直接测量的皮质醇浓度（P = 0.06）外，Hal基因对所研究的变量没有重大影响（NN = 66.8 nM，Nn = 61.4 nM，nn = 69.4 nM）。然而，每种Hal基因型的反应不同，这取决于猪是否经历过运输。运输两周后，NN猪的皮质醇浓度高于未运输的动物，而nn动物的反应则相反；Nn猪在这方面没有差异。屠宰时，运输（12周龄）对皮质醇和CBG的影响仍然明显。与未运输的猪相比，NN猪的皮质醇和CBG浓度升高（分别为P≤0.05，P = 0.08），而nn猪的浓度则趋于较低（分别为P = 0.17，P = 0.07）。运输的猪在屠宰时的受体浓度低于未运输的猪（P≤0.01）。然而，运输时给予安哌齐特的猪的受体浓度与未运输的猪相当。我们的研究确凿地表明，运输应激对皮质醇、CBG和糖皮质激素受体浓度有长期影响。此外，安哌齐特对皮质醇和受体浓度有长期影响。
