Blackshaw J K, Blackshaw A W
Department of Farm Animal Medicine and Production, University of Queensland, St Lucia, Australia.
Vet Res Commun. 1989;13(4):265-71. doi: 10.1007/BF00420834.
Blood and saliva samples were taken from groups of pigs maintained in intensive conditions. Multiple samples were taken from two unrestrained pregnant sows fitted with jugular cannulae. Single samples were taken from groups (mixed gilts and entire males; 70-90 kg) which were lightly exercised (7) or restrained (12). The rate of salivary secretion was low and collection of adequate samples took 5 min; in a number of pigs no saliva could be obtained. In order to stimulate salivary secretion, pigs (70-80 kg) were injected with pilocarpine nitrate (25 mg, subcutaneous) which produced a copious flow of saliva persisting for at least 15 min. Resting sows had higher mean levels of cortisol in plasma, ultrafiltrate and saliva than the other groups, which did not differ from each other. Within the pilocarpine group, males had a higher ultrafiltrate level of cortisol than females. In most instances salivary cortisol was significantly greater than ultrafiltrate cortisol. Ultrafiltrate and plasma cortisol were highly correlated (r = 0.883) but this correlation was low in the presence of pilocarpine (r = 0.260). Salivary cortisol was poorly correlated with either plasma (r = 0.167) or ultrafiltrate cortisol (r = 0.278) and the correlation with plasma was even lower following the administration of pilocarpine (r = 0.086). It was concluded that salivary estimates of cortisol in the pig were not usefully correlated with levels of ultrafiltrate (free) cortisol.
从集约化饲养的猪群中采集血液和唾液样本。从两只装有颈静脉插管的未受约束的怀孕母猪身上采集了多个样本。从轻度运动（7头）或受约束（12头）的猪群（混合后备母猪和成年公猪；70 - 90千克）中采集单个样本。唾液分泌速率较低，采集足够的样本需要5分钟；在一些猪身上无法获得唾液。为了刺激唾液分泌，给70 - 80千克的猪注射硝酸毛果芸香碱（25毫克，皮下注射），这会产生持续至少15分钟的大量唾液分泌。静息母猪的血浆、超滤液和唾液中的皮质醇平均水平高于其他组，而其他组之间没有差异。在毛果芸香碱组中，公猪的超滤液皮质醇水平高于母猪。在大多数情况下，唾液皮质醇显著高于超滤液皮质醇。超滤液和血浆皮质醇高度相关（r = 0.883），但在使用毛果芸香碱时这种相关性较低（r = 0.260）。唾液皮质醇与血浆（r = 0.167）或超滤液皮质醇（r = 0.278）的相关性都很差，并且在使用毛果芸香碱后与血浆的相关性更低（r = 0.086）。得出的结论是，猪唾液中皮质醇的估计值与超滤液（游离）皮质醇水平没有有效相关性。
