NMPA Key Laboratory for Testing Technology of Pharmaceutical Microbiology, Shanghai Institute for Food and Drug Control, Shanghai 201203, China.
Department of Chemistry, and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
Analyst. 2021 Jun 28;146(13):4146-4153. doi: 10.1039/d1an00335f.
Bacterial infections cause considerable morbidity and expensive healthcare costs. The prescription of broad-spectrum antimicrobial drugs results in failure of treatment or overtreatment and exacerbates the spread of multidrug-resistant pathogens. There is an emergent demand for rapid and accurate methods to identify pathogens and conduct personalized therapy. Here, we develop a herringbone microfluidic chip integrated with vancomycin modified magnetic beads (herringbone-VMB microchip) to enrich pathogens. The enriched pathogens are identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The herringbone-VMB microchip applies passive mixing of bacterial samples by generating microvortices, which significantly enhances the interaction between bacteria and vancomycin modified magnetic beads and leads to more efficient enrichment compared to in-tube extraction. Four common pathogens in urinary tract infections are utilized to validate the method, and the capture efficiency of the bacteria from urine is up to 90%. The whole procedure takes 1.5 hours from enrichment to identification. This method shows potential in shortening the turnaround time in the clinical diagnosis of bacterial infections.
细菌感染会导致相当大的发病率和昂贵的医疗保健费用。广谱抗菌药物的处方会导致治疗失败或过度治疗，并加剧多药耐药病原体的传播。因此，人们迫切需要快速、准确的方法来鉴定病原体并进行个性化治疗。在这里，我们开发了一种人字形微流控芯片，该芯片与万古霉素修饰的磁珠（人字形-VMB 微芯片）集成，用于富集病原体。通过基质辅助激光解吸电离飞行时间质谱法对富集的病原体进行鉴定。人字形-VMB 微芯片通过产生微涡流来实现细菌样本的被动混合，这显著增强了细菌与万古霉素修饰磁珠之间的相互作用，与管内提取相比，实现了更高效的富集。利用四种常见的尿路感染病原体对该方法进行了验证，从尿液中捕获细菌的效率高达 90%。从富集到鉴定，整个过程耗时 1.5 小时。该方法有望缩短细菌感染临床诊断的周转时间。
