Hleba Lukas, Hlebova Miroslava, Kovacikova Eva, Kovacik Anton
Institute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. Andreja Hlinku 2, 949 76 Nitra, Slovakia.
Department of Biology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Nám. J. Herdu 2, 917 01 Trnava, Slovakia.
Microorganisms. 2023 Apr 13;11(4):1018. doi: 10.3390/microorganisms11041018.
Rapid identification of beta-lactamase-producing strains of plays key role in diagnostics in clinical microbiology. Therefore, the aim of this study was the rapid determination of beta-lactamase's presence in isolates via indirect detection of degradation ampicillin products using MALDI-TOF MS. isolates were subjected to antibiotic resistance testing using disk diffusion and MIC methodologies. Beta-lactamase activity was tested using MALDI-TOF MS, and results were compared to spectral analysis of alkaline hydrolysis. Resistant and susceptible strains of were distinguished, and strains with a high MIC level were identified as beta-lactamase-producing. Results indicate that MALDI-TOF mass spectrometry is also suitable for the rapid identification of beta-lactamase-producing . This observation and confirmation can accelerate identification of beta-lactamase strains of in clinical microbiology, which can have an impact on health in general.
快速鉴定产β-内酰胺酶菌株在临床微生物学诊断中起着关键作用。因此，本研究的目的是通过使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）间接检测氨苄西林降解产物，快速测定分离株中β-内酰胺酶的存在情况。使用纸片扩散法和最低抑菌浓度（MIC）方法对分离株进行抗生素耐药性测试。使用MALDI-TOF MS检测β-内酰胺酶活性，并将结果与碱水解的光谱分析进行比较。区分了肺炎克雷伯菌的耐药和敏感菌株，并将高MIC水平的菌株鉴定为产β-内酰胺酶菌株。结果表明，MALDI-TOF质谱也适用于快速鉴定产β-内酰胺酶的肺炎克雷伯菌。这一观察结果和确认可以加速临床微生物学中肺炎克雷伯菌β-内酰胺酶菌株的鉴定，这总体上可能对健康产生影响。
