Key Laboratory of Organosilicon Chemistry and Material Technology, Hangzhou Normal University, Hangzhou, P. R. China.
J Sep Sci. 2021 Jul;44(14):2856-2861. doi: 10.1002/jssc.202100218. Epub 2021 May 28.
Phytic acid, the principal storage form of phosphorus in wheat, plays both beneficial and antinutrient functions for human being, and its analytical method still needs further development. In this work, we have developed a new method for the determination of phytic acid in wheat products based on derivatization with (trimethylsilyl)diazomethane in combination with liquid chromatography-mass spectrometry analysis. Methyl esterification greatly decreased the polarity and the acidity of phytic acid, and thus the corresponding derivative can be easily analyzed by liquid chromatography-mass spectrometry under common conditions. Furthermore, treatment with cation exchange resin removed the polyvalent metal ions in the solutions, and thus derivatization of phytic acid can be achieved efficiently and completely. The standard curve for phytic acid has been well established in the linear range of 0.5-100 ng/mL with squared correlation coefficient more than 0.999 and the quantification limit of 0.25 ng/mL. The phytic acid content varies greatly in different wheat products, ranging from 153.5 to 17299.0 μg/g.
植酸是小麦中磷的主要储存形式，对人类既有有益的功能，也有抗营养功能，其分析方法仍需进一步发展。在这项工作中，我们开发了一种基于（三甲基硅基）重氮甲烷衍生化结合液相色谱-质谱分析测定小麦制品中植酸的新方法。甲酯化极大地降低了植酸的极性和酸度，因此在常见条件下，相应的衍生物可以很容易地通过液相色谱-质谱分析。此外，用阳离子交换树脂处理溶液中的多价金属离子，可以有效地、完全地进行植酸的衍生化。植酸的标准曲线在 0.5-100ng/mL 的线性范围内得到了很好的建立，相关系数大于 0.999，定量限为 0.25ng/mL。不同小麦制品中的植酸含量差异很大，范围从 153.5 到 17299.0μg/g。
