用于通过高效液相色谱法（HPLC）示差折光检测法测定植酸的小麦样品处理方法的优化。

Optimization of the treatment of wheat samples for the determination of phytic acid by HPLC with refractive index detection.

作者信息

Amaro Rosa, Murillo Miguel, González Zurima, Escalona Andrés, Hernández Luís

机构信息

Universidad Central de Venezuela, Facultad de Ciencias, Escuela de Química, Centro de Química Analítica, Laboratorio de Métodos Cromatográficos, Apartado 47102, Caracas 1041-A, Venezuela.

出版信息

J AOAC Int. 2009 May-Jun;92(3):873-8.

PMID:19610380

Abstract

The treatment of wheat samples was optimized before the determination of phytic acid by high-performance liquid chromatography with refractive index detection. Drying by lyophilization and oven drying were studied; drying by lyophilization gave better results, confirming that this step is critical in preventing significant loss of analyte. In the extraction step, washing of the residue and collection of this water before retention of the phytates in the NH2 Sep-Pak cartridge were important. The retention of phytates in the NH2 Sep-Pak cartridge and elimination of the HCI did not produce significant loss (P = 0.05) in the phytic acid content of the sample. Recoveries of phytic acid averaged 91%, which is a substantial improvement with respect to values reported by others using this methodology.

摘要

在用示差折光检测的高效液相色谱法测定植酸之前，对小麦样品的处理进行了优化。研究了冻干和烘干两种干燥方式；结果表明冻干效果更好，证实了这一步骤对于防止分析物的大量损失至关重要。在提取步骤中，在将植酸盐保留在NH2 Sep-Pak柱之前洗涤残渣并收集此洗涤水很重要。植酸盐保留在NH2 Sep-Pak柱中以及去除HCl不会导致样品中植酸含量出现显著损失（P = 0.05）。植酸的回收率平均为91%，相对于其他使用该方法的人所报告的值有了显著提高。