病毒 RNA 修饰的检测与功能：生物学和医学的新视角。
Detection and functions of viral RNA modifications: perspectives in biology and medicine.
机构信息
Université de Lorraine, CNRS, INSERM, IBSLor (UMS2008/US40), Epitranscriptomics and RNA Sequencing Core Facility, F54000 Nancy, France.
Université de Lorraine, CNRS, INSERM, IBSLor (UMS2008/US40), Epitranscriptomics and RNA Sequencing Core Facility, F54000 Nancy, France, Université de Lorraine, CNRS, IMoPA (UMR7365), F54000 Nancy, France.
出版信息
Virologie (Montrouge). 2021 Apr 1;25(2):5-20. doi: 10.1684/vir.2021.0884.
Viral RNAs (either derived from DNA viruses and genomic/mRNAs of RNA viruses) produced and replicated in eukaryotic cells are exposed to the activity of host cell RNA modification machinery. Moreover, some complex viruses encode their own RNA modification enzymes, generally cap-related mG-and 2'-O-methyltransferases whose expression allows specific modification of viral transcripts and modulation of viral RNA recognition by host restriction systems. Here we review current achievements in the detection of viral RNA modifications by liquid chromatography coupled to mass spectrometry (LC-MS) and deep sequencing-based approaches. The presence, origin and characterized functions of RNA modifications in viral RNAs are discussed.
真核细胞中产生和复制的病毒 RNA（源自 DNA 病毒和 RNA 病毒的基因组/mRNA）会受到宿主细胞 RNA 修饰机制的影响。此外，一些复杂的病毒会编码自己的 RNA 修饰酶，通常是与帽子结构相关的 mG 和 2'-O-甲基转移酶，其表达可以特异性修饰病毒转录本，并调节宿主限制系统对病毒 RNA 的识别。在这里，我们综述了通过液相色谱-质谱联用（LC-MS）和基于深度测序的方法检测病毒 RNA 修饰的最新进展。讨论了病毒 RNA 中 RNA 修饰的存在、起源和特征功能。
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