Marchand Virginie, Motorin Yuri
Université de Lorraine, CNRS, Inserm, IBSLor (UMS2008/US40), Epitranscriptomics and RNA Sequencing Core Facility, F54000 Nancy, France.
Université de Lorraine, CNRS, Inserm, IBSLor (UMS2008/US40), Epitranscriptomics and RNA Sequencing Core Facility, F54000 Nancy, France, Université de Lorraine, CNRS, IMoPA (UMR7365), F54000 Nancy, France.
Virologie (Montrouge). 2021 Apr 1;25(2):93-110. doi: 10.1684/vir.2021.0899.
Viral RNAs (either derived from DNA viruses and genomic/mRNAs of RNA viruses) produced and replicated in eukaryotic cells are exposed to the activity of host cell RNA modification machinery. Moreover, some complex viruses encode their own RNA modification enzymes, generally cap-related mG-and 2'-O-methyltransferases whose expression allows specific modification of viral transcripts and modulation of viral RNA recognition by host restriction systems. Here we review current achievements in the detection of viral RNA modifications by liquid chromatography coupled to mass spectrometry (LC-MS) and deep sequencing-based approaches. The presence, origin and characterized functions of RNA modifications in viral RNAs are discussed.
在真核细胞中产生并复制的病毒RNA（无论是源自DNA病毒还是RNA病毒的基因组/信使RNA）都会受到宿主细胞RNA修饰机制的作用。此外，一些复杂病毒会编码自身的RNA修饰酶，通常是与帽相关的mG和2'-O-甲基转移酶，其表达允许对病毒转录本进行特定修饰，并调节宿主限制系统对病毒RNA的识别。在此，我们综述了通过液相色谱-质谱联用（LC-MS）和基于深度测序的方法检测病毒RNA修饰的当前成果。讨论了病毒RNA中RNA修饰的存在、来源和已表征的功能。
