Programa de Pos Graduacao Medicina Animal: Equinos, Faculdade de Veterinaria, Universidade Federal do Rio Grande do Sul, Rio Grande do Sul, Brazil.
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Cryo Letters. 2020 Jan-Feb;41(1):6-12.
Epididymal sperm cryopreservation represents the ultimate option to preserve spermatozoa of valuable stallions.
The study aims to evalute whether single layer centrifugation (SLC) prior to cryopreservation or after post-thawing improves the quality of stallion epididymal sperm.
Epididymal sperms of stallions were harvested (N=20). Sperm samples were subjected to treatments: conventional centrifugation, SLC prior to cryopreservation (SLC-PC) or SLC post-thaw (SLC+). All samples were cryopreserved, thawed and evaluated. SLC+ were thawed, single layer cenrifuged and resuspended in freezing extender (SLC+F) or cooling extender (SLC+C). Total motility, progressive motility, morphology, mitochondrial functionality, membrane integrity and DNA integrity were evaluated.
SLC-PC and SLC+F yielded higher total motility, while SLC+F yielded the highest progressive motility. Mitochondrial functionality was significantly higher in all SLC groups. Membrane integrity was higher in SLC-PC. The percentage of morphologically normal spermatozoa was higher in SLC-PC and SLC+F.
SLC prior to cryopreservation or post-thaw improves the quality of stallion epididymal spermatozoa. When SLC is performed post-thaw, freezing extender is the best medium to resuspend the pelleted semen.
附睾精子冷冻保存是保存优质种马精子的最终选择。
本研究旨在评估冷冻保存前或解冻后单层离心（SLC）是否能提高种马附睾精子的质量。
采集种马附睾精子（N=20）。精子样本进行以下处理：常规离心、冷冻保存前 SLC（SLC-PC）或解冻后 SLC（SLC+）。所有样本均进行冷冻保存、解冻和评估。SLC+解冻后，单层离心并悬浮于冷冻保存液（SLC+F）或冷却液（SLC+C）中。评估总活力、前向运动活力、形态、线粒体功能、膜完整性和 DNA 完整性。
SLC-PC 和 SLC+F 组总活力较高，而 SLC+F 组前向运动活力最高。所有 SLC 组的线粒体功能均显著提高。SLC-PC 组的膜完整性较高。SLC-PC 和 SLC+F 组形态正常精子的比例较高。
冷冻保存前或解冻后进行 SLC 可提高种马附睾精子的质量。解冻后进行 SLC 时，冷冻保存液是沉淀精液的最佳悬浮介质。
