胰蛋白酶对芜菁花叶病毒（TuMV）及其他马铃薯Y病毒属病毒纯化的有益作用。

A beneficial effect of trypsin on the purification of turnip mosaic virus (TuMV) and other potyviruses.

作者信息

Thompson S, Fraser R S, Barnden K L

机构信息

Biochemistry and Molecular Biology Department, Institute of Horticultural Research, Wellesbourne, Warwick, U.K.

出版信息

J Virol Methods. 1988 May;20(1):57-64. doi: 10.1016/0166-0934(88)90040-7.

DOI:10.1016/0166-0934(88)90040-7

PMID:3397402

Abstract

The effects of treatment with trypsin during the purification of turnip mosaic virus (TuMV), on virus yield, infectivity and integrity of virus coat protein were examined. Trypsin increased yield markedly, and at a low concentration, increased infectivity. These effects were probably due to reduced aggregation of virus particles. At higher concentrations of trypsin, there was some degradation of virus coat protein, and infectivity was reduced. Treatment with trypsin at the optimum concentration can significantly improve purification of TuMV; more limited experiments suggest that it can also be applied to other potyviruses.

摘要

研究了在芜菁花叶病毒（TuMV）纯化过程中用胰蛋白酶处理对病毒产量、感染性及病毒外壳蛋白完整性的影响。胰蛋白酶显著提高了产量，且在低浓度时增加了感染性。这些效应可能是由于病毒粒子聚集减少所致。在较高浓度的胰蛋白酶作用下，病毒外壳蛋白有一些降解，感染性降低。以最佳浓度用胰蛋白酶处理可显著改善TuMV的纯化；更多有限的实验表明它也可应用于其他马铃薯Y病毒属病毒。