Wang D, Hayes I M, Maule A J
John Innes Centre for Plant Science Research, Norwich, U.K.
J Virol Methods. 1992 Mar;36(3):223-30. doi: 10.1016/0166-0934(92)90053-g.
An efficient protocol for the purification of pea seed-borne mosaic potyvirus (PSbMV) particles was developed. This led to the purification of 10 PSbMV isolates by a single procedure. Virus aggregation during purification did not occur and consequently, high virus yield was consistently obtained. The virus thus purified was suitable for preparing viral genomic RNA, although conventional methods for RNA extraction resulted in RNA degradation. An alternative method was adopted which yielded reproducibly full length and infectious RNA. This was applied to three isolates of PSbMV and the RNA used to direct complementary DNA synthesis which in turn yielded nearly full length cDNA products.
开发了一种高效的纯化豌豆种传花叶马铃薯Y病毒（PSbMV）颗粒的方法。通过单一程序纯化出了10种PSbMV分离株。纯化过程中未发生病毒聚集，因此始终能获得高产率的病毒。如此纯化得到的病毒适合用于制备病毒基因组RNA，不过传统的RNA提取方法会导致RNA降解。于是采用了一种替代方法，该方法可重复产生全长且具有感染性的RNA。此方法应用于3种PSbMV分离株，所获RNA用于指导互补DNA合成，进而产生了近乎全长的cDNA产物。
