Millman I, Glass R G
Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
J Virol Methods. 1988 May;20(1):83-7. doi: 10.1016/0166-0934(88)90043-2.
'Ausria II' polystyrene beads (Abbott Labs, N. Chicago) are reacted with woodchuck serum positive for WHsAg in a dilution predetermined by titration. This modified bead is used in a blocking assay to detect the presence of antibody to the surface antigen of woodchuck hepatitis virus (anti-WHs). Serum containing woodchuck anti-WHs and commercial horseradish peroxidase (HRP) labeled anti-HBs are sequentially added. A drop in optical density at 492 nm of 50% or more due to the blocking of HRP conjugated anti-HBs by anti-WHs compared with a control (negative woodchuck serum) is a measure of anti-WHs. The ease and simplicity of converting readily available 'Ausria II' beads to specific reagents for detecting anti-WHs should be welcomed by investigators studying WHV. The method described is both sensitive and reproducible.
将“Ausria II”聚苯乙烯珠（雅培实验室，北芝加哥）与经滴定预先确定稀释度的、对土拨鼠表面抗原（WHsAg）呈阳性的土拨鼠血清反应。这种经过改良的珠子用于阻断试验，以检测土拨鼠肝炎病毒表面抗原抗体（抗 - WHs）的存在。依次加入含有土拨鼠抗 - WHs的血清和商业化辣根过氧化物酶（HRP）标记的抗 - HBs。与对照（阴性土拨鼠血清）相比，由于抗 - WHs阻断了HRP偶联的抗 - HBs，在492nm处光密度下降50%或更多，即为抗 - WHs的一种检测方法。对于研究土拨鼠肝炎病毒（WHV）的研究人员来说，将现成的“Ausria II”珠子转化为检测抗 - WHs的特异性试剂的简便性应该是值得欢迎的。所描述的方法既灵敏又可重复。
