Ponzetto A, Cote P J, Ford E C, Engle R, Cicmanec J, Shapiro M, Purcell R H, Gerin J L
Virus Res. 1985 Jun;2(4):301-15. doi: 10.1016/0168-1702(85)90027-9.
Solid-phase radioimmunoassays for woodchuck hepatitis virus core antigen (WHcAg) and antibody (anti-WHc) were developed. WHcAg in woodchuck liver homogenates was characterized by ultracentrifugation in CsCl gradients; both heavy (1.35 g/cm3) and light (1.31 g/cm3) cores were obtained from the liver of an animal during acute WHV infection, which is consistent with observations in hepatitis B virus infection in man. Endpoint titers of anti-WHc were higher in chronic WHV carriers than in animals recovered from acute infections. Both IgM and IgG anti-WHc antibodies were produced by infected woodchucks. A survey of colony woodchucks demonstrated that 88/89 animals having one or more markers of past or ongoing WHV infection were positive for anti-WHc. Thus, serum anti-WHc appears to be a sensitive marker of WHV infection.
开发了用于土拨鼠肝炎病毒核心抗原（WHcAg）和抗体（抗-WHc）的固相放射免疫测定法。通过在CsCl梯度中进行超速离心对土拨鼠肝脏匀浆中的WHcAg进行了表征；在急性土拨鼠肝炎病毒（WHV）感染期间，从一只动物的肝脏中获得了重（1.35 g/cm³）轻（1.31 g/cm³）两种核心，这与人乙型肝炎病毒感染中的观察结果一致。慢性WHV携带者中抗-WHc的终点滴度高于从急性感染中恢复的动物。受感染的土拨鼠会产生IgM和IgG抗-WHc抗体。对圈养土拨鼠的一项调查表明，88/89只具有过去或正在进行的WHV感染一种或多种标志物的动物抗-WHc呈阳性。因此，血清抗-WHc似乎是WHV感染的一个敏感标志物。
