Aly Shaza H, Elissawy Ahmed M, Allam Ahmed E, Farag Shaimaa M, Eldahshan Omayma A, Elshanawany Mohamed A, Singab Abdel Nasser B
Department of Pharmacognosy, Faculty of Pharmacy, Badr University in Cairo, Cairo, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt.
Nat Prod Res. 2022 Jun;36(11):2722-2734. doi: 10.1080/14786419.2021.1919108. Epub 2021 May 11.
Phytochemical investigation of alkaloid fraction led to isolation of one new quinolizidine alkaloid 13-methoxyanagyrine together with six known ones . The insecticidal activity of 70% methanol extract of leaves of , and the isolated alkaloids were assessed against 3 instar larvae of (Diptera: Culicidae) using different concentrations and mortality rate was recorded. extract showed highest mortality rate with median lethal concentration LC 3.11 ppm after 24 h and 0.66 ppm after 48 h and anagyrine exhibited remarkably insecticidal activity with LC value of 3.42 ppm after 24 h of exposure. Additionally, cytotoxic activity of alkaloid fraction of , and isolated alkaloids was also studied using crystal violet assay against MCF-7 and HEPG-2 cell lines. Anagyrine exhibited IC values of 27.3 ± 0.7 and 30.2 ± 0.9 µg/mL against MCF-7 and HEPG-2 cancer cells, respectively.
对生物碱部分进行植物化学研究，分离出一种新的喹诺里西啶生物碱13-甲氧基纳吉宁以及六种已知生物碱。使用不同浓度，对[植物名称1]、[植物名称2]和[植物名称3]叶片的70%甲醇提取物以及分离出的生物碱对致倦库蚊（双翅目：蚊科）3龄幼虫的杀虫活性进行了评估，并记录了死亡率。[植物名称1]提取物显示出最高的死亡率，24小时后的半数致死浓度LC为3.11 ppm，48小时后为0.66 ppm，纳吉宁在暴露24小时后LC值为3.42 ppm，表现出显著的杀虫活性。此外，还使用结晶紫测定法对[植物名称1]、[植物名称2]的生物碱部分以及分离出的生物碱对MCF-7和HEPG-2细胞系的细胞毒性活性进行了研究。纳吉宁对MCF-7和HEPG-2癌细胞的IC值分别为27.3±0.7和30.2±0.9 μg/mL。
