Neuroprotective effects of , leaves and formononetin on scopolamine-induced dementia.

Five flavonoids were isolated from the ethyl acetate fraction of leaves of ; formononetin , 5-hydroxy-4'-methoxyflavone , genistein , 5-hydroxy-8-(1-hydroxy-1-methyl-ethyl)-2-(4-hydroxyphenyl)-4-furo-[2, 3-]-chromen-4-one and ononin . Additionally, LC-ESI-MS/MS analysis of the ethyl acetate fraction of leaves had led to tentative identification of eighteen compounds. Formononetin, and leaves methanolic extract were evaluated for their neuroprotective activity where formononetin and showed promising neuroprotective activity with reduction in acetylcholine esterase (AchE) enzyme activity and elevation of acetylcholine (Ach) and glutathione(GSH) brain levels and attenuation of dopamine (DA), nor-adrenaline (NA) and malonedialdehyde (MDA) brain level significantly, However leaves methanolic extract didn't attenuate the AchE enzyme activity, DA and NA brain levels.