Department of Medical Sciences, General Graduate School, Soonchunhyang University, Asan, South Korea.
Department of Medical Sciences, General Graduate School, Soonchunhyang University, Asan, South Korea; Department of Medical Biotechnology, Soonchunhyang University, Asan, South Korea.
Mech Ageing Dev. 2021 Jul;197:111498. doi: 10.1016/j.mad.2021.111498. Epub 2021 May 8.
Phosphatidylethanolamine is a major component of phospholipids with both structural and metabolic functions in cells. Previous studies have revealed that phosphatidylethanolamine can modulate autophagy with a protective effect against age-related diseases. We examined the effect of dietary supplementation with phosphatidylethanolamine on stress response and aging in Caenorhabditis elegans. Phosphatidylethanolamine increased resistance to oxidative stress without effect on heat stress or ultraviolet irradiation. Both mean and maximum lifespans were significantly increased by phosphatidylethanolamine while fertility was reduced as a trade-off. Age-related decline of muscle function was delayed in animals treated with phosphatidylethanolamine. Supplementation with phosphatidylethanolamine suppressed toxic effect of amyloid β and high-glucose diet. Increased ROS levels and induction of stress-responsive genes after dietary supplementation with phosphatidylethanolamine suggest that anti-oxidative stress and anti-aging effects of phosphatidylethanolamine might be though hormesis. Genetic analysis using long-lived mutants and knockdown by RNAi revealed that the lifespan-extending effect of phosphatidylethanolamine overlapped with that of reduced insulin/IGF-1-like signaling and required DAF-16, a downstream transcription factor known to regulate the expression of many stress-responsive genes. These findings indicate that phosphatidylethanolamine has anti-oxidative stress and anti-aging activities with its underlying mechanisms involving hormesis and reduced insulin/IGF-1-like signaling in C. elegans.
磷脂酰乙醇胺是磷脂的主要成分，具有细胞结构和代谢功能。先前的研究表明，磷脂酰乙醇胺可以调节自噬，对与年龄相关的疾病具有保护作用。我们研究了饮食补充磷脂酰乙醇胺对秀丽隐杆线虫应激反应和衰老的影响。磷脂酰乙醇胺增加了对氧化应激的抗性，而对热应激或紫外线照射没有影响。磷脂酰乙醇胺显著延长了平均寿命和最大寿命，同时降低了生育能力作为权衡。用磷脂酰乙醇胺处理的动物肌肉功能的衰老速度延迟。磷脂酰乙醇胺补充剂抑制了淀粉样β和高糖饮食的毒性作用。饮食补充磷脂酰乙醇胺后 ROS 水平升高和应激响应基因的诱导表明，磷脂酰乙醇胺的抗氧化应激和抗衰老作用可能是通过应激反应实现的。使用长寿突变体的遗传分析和 RNAi 敲低表明，磷脂酰乙醇胺的寿命延长作用与降低胰岛素/IGF-1 样信号重叠，并且需要 DAF-16，这是一种已知调节许多应激响应基因表达的下游转录因子。这些发现表明，磷脂酰乙醇胺在秀丽隐杆线虫中具有抗氧化应激和抗衰老活性，其潜在机制涉及应激反应和降低胰岛素/IGF-1 样信号。
