Translational Research Unit, Veterinary Teaching Hospital, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
One Health Research Center, Hokkaido University, Sapporo, Japan.
Sci Rep. 2021 May 11;11(1):10005. doi: 10.1038/s41598-021-89279-0.
DNA methylation plays important functions in gene expression regulation that is involved in individual development and various diseases. DNA methylation has been well studied in human and model organisms, but only limited data exist in companion animals like dog. Using methylation-sensitive restriction enzyme-based next generation sequencing (Canine DREAM), we obtained canine DNA methylation maps of 16 somatic tissues from two dogs. In total, we evaluated 130,861 CpG sites. The majority of CpG sites were either highly methylated (> 70%, 52.5-64.6% of all CpG sites analyzed) or unmethylated (< 30%, 22.5-28.0% of all CpG sites analyzed) which are methylation patterns similar to other species. The overall methylation status of CpG sites across the 32 methylomes were remarkably similar. However, the tissue types were clearly defined by principle component analysis and hierarchical clustering analysis with DNA methylome. We found 6416 CpG sites located closely at promoter region of genes and inverse correlation between DNA methylation and gene expression of these genes. Our study provides basic dataset for DNA methylation profiles in dogs.
DNA 甲基化在基因表达调控中发挥着重要作用，涉及个体发育和各种疾病。DNA 甲基化在人类和模式生物中得到了很好的研究，但在狗等伴侣动物中只有有限的数据。我们使用基于甲基敏感限制性内切酶的下一代测序（Canine DREAM），从两只狗中获得了 16 种体细胞组织的犬 DNA 甲基化图谱。总共评估了 130861 个 CpG 位点。大多数 CpG 位点要么高度甲基化（>70%，分析的所有 CpG 位点的 52.5-64.6%），要么非甲基化（<30%，分析的所有 CpG 位点的 22.5-28.0%），这与其他物种的甲基化模式相似。32 个甲基组中 CpG 位点的整体甲基化状态非常相似。然而，通过主成分分析和层次聚类分析，组织类型与 DNA 甲基化组明显相关。我们发现 6416 个 CpG 位点位于基因启动子区域附近，这些基因的 DNA 甲基化与基因表达呈负相关。我们的研究为犬类 DNA 甲基化图谱提供了基础数据集。
