Zhu Lihuan, Zhou Dongsheng, Guo Tianxing, Chen Wenshu, Ding Yun, Li Wujing, Huang Yangyun, Huang Jianyuan, Pan Xiaojie
Department of Thoracic Surgery, Fujian Provincial Hospital, Fuzhou, China.
Department of Radiology, Fujian Provincial Hospital, Fuzhou, China.
J Cancer. 2021 Apr 2;12(11):3291-3298. doi: 10.7150/jca.56218. eCollection 2021.
Lung cancer is a malignant tumor in mammary gland epithelium with high morbidity and mortality among women worldwide. Long noncoding RNA GAS5 (GAS5) has been proved to be closely related with tumor progression. However, the influence of GAS5 on lung cancer and the specific mechanism remain unclear. Cell invasion, cell migration, cell apoptosis and cell cycle were investigated after transfection with pcDNA-GAS5 and sh-GAS5. Sizes of tumors were measured by establishing transplanted tumor model . E-cadherin and N-cadherin expressions were investigated. Cell invasion and migration were inhibited markedly in GAS5 overexpressed cell line. Cell cycle results indicated that the percentage of S-phase cells was increased, and G2-phase was reduced in the GAS5 overexpression cell line. Tumor size was suppressed obviously after GAS5 overexpression treatment. GAS5 markedly inhibited the expression of E-cadherin and induced the expression of N-cadherin. GAS5 overexpression significantly inhibited lung cancer cell proliferation by increasing the E-cadherin and decreasing N-cadherin. These findings provide novel evidence that GAS5 can be viewed as an anti-lung cancer agent through affecting EMT pathway.
肺癌是一种发生于乳腺上皮的恶性肿瘤，在全球女性中发病率和死亡率都很高。长链非编码RNA GAS5（GAS5）已被证明与肿瘤进展密切相关。然而，GAS5对肺癌的影响及其具体机制仍不清楚。在用pcDNA-GAS5和sh-GAS5转染后，研究了细胞侵袭、细胞迁移、细胞凋亡和细胞周期。通过建立移植瘤模型测量肿瘤大小。检测E-钙黏蛋白和N-钙黏蛋白的表达。在GAS5过表达的细胞系中，细胞侵袭和迁移明显受到抑制。细胞周期结果表明，在GAS5过表达细胞系中，S期细胞百分比增加，G2期细胞减少。GAS5过表达处理后，肿瘤大小明显受到抑制。GAS5显著抑制E-钙黏蛋白的表达并诱导N-钙黏蛋白的表达。GAS5过表达通过增加E-钙黏蛋白和减少N-钙黏蛋白显著抑制肺癌细胞增殖。这些发现提供了新的证据，表明GAS5可通过影响上皮-间质转化（EMT）途径被视为一种抗肺癌药物。
