Fu Longfei, Monro Alexandre K, Yang Tiange, Wen Fang, Pan Bo, Xin Zibing, Zhang Zhixiang, Wei Yigang
Laboratory of Systematic Evolution and Biogeography of Woody Plants, College of Nature Conservation, Beijing Forestry University, Beijing, China.
Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, China.
PeerJ. 2021 Apr 19;9:e11148. doi: 10.7717/peerj.11148. eCollection 2021.
L.F. Fu, A.K. Monro & Y.G. Wei, a new species from Guangxi, China is described and illustrated. Morphologically, is most similar to from which it differs by having smaller size of leaf laminae, fewer and smaller staminate peduncle bracts, longer pistillate peduncle bracts and a larger achene. This result is supported by the molecular evidence. The phylogenetic position of the new species within is evaluated using three DNA regions, ITS, and , for 107 taxa of s.l. (including ). Bayesian inference (BI) and maximum likelihood (ML) analyses each recovered the same strongly supported tree topologies, indicating that is a member of the core clade and sister to . Along with the phylogenetic studies, plastid genome and ribosomal DNA (rDNA) sequences of the new species are assembled and annotated. The plastid genome is 150,398 bp in length and comprises two inverted repeats (IRs) of 24,688 bp separated by a large single-copy of 83,919 bp and a small single-copy of 17,103 bp. A total of 113 functional genes are recovered, comprising 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. The rDNA is 5,804 bp in length and comprised the 18S ribosomal RNA partial sequence (1,809 bp), internal transcribed spacer 1 (213 bp), 5.8S ribosomal RNA (164 bp), internal transcribed spacer 2 (248 bp) and 26S ribosomal RNA partial sequence (3,370 bp). In addition, the chromosome number of is observed to be 2 = 26, suggesting that the species is diploid. Given a consistent relationship between ploidy level and reproductive system in , the new species is also considered to be sexually reproducing. Our assessment of the extinction threat for is that it is Endangered (EN) according to the criteria of the International Union for Conservation of Nature.
描述并图示了来自中国广西的新物种傅立峰、A.K. 蒙罗和魏永刚。在形态上，它与[某物种]最为相似，但不同之处在于叶片较小、雄花序梗苞片较少且较小、雌花序梗苞片较长以及瘦果较大。这一结果得到了分子证据的支持。利用三个DNA区域，即ITS、[某区域]和[某区域]，对广义[某属]的107个分类群（包括[某物种]）进行评估，以确定该新物种在[某属]中的系统发育位置。贝叶斯推断（BI）和最大似然（ML）分析各自得到了相同的、得到强烈支持的树形拓扑结构，表明[某物种]是核心[某属]分支的成员，并且是[某物种]的姐妹种。随着系统发育研究的开展，新物种的质体基因组和核糖体DNA（rDNA）序列得以组装和注释。质体基因组长度为150,398 bp，由两个24,688 bp的反向重复序列（IRs）组成，中间被一个83,919 bp的大单拷贝和一个17,103 bp的小单拷贝隔开。总共鉴定出113个功能基因，包括79个蛋白质编码基因、30个tRNA基因和4个rRNA基因。rDNA长度为5,804 bp，由18S核糖体RNA部分序列（1,809 bp）、内部转录间隔区1（213 bp）、5.8S核糖体RNA（164 bp）、内部转录间隔区2（248 bp）和26S核糖体RNA部分序列（3,370 bp）组成。此外，观察到[某物种]的染色体数为2n = 26，表明该物种是二倍体。鉴于[某属]中倍性水平与繁殖系统之间存在一致关系，该新物种也被认为是有性繁殖的。根据国际自然保护联盟的标准，我们对[某物种]灭绝威胁的评估是它处于濒危（EN）状态。
