使用 BioID 技术鉴定 RAS 相互作用组。
Using BioID to Characterize the RAS Interactome.
机构信息
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC, USA.
Department of Radiation Oncology, Duke University Medical Center, Durham, NC, USA.
出版信息
Methods Mol Biol. 2021;2262:271-280. doi: 10.1007/978-1-0716-1190-6_16.
Identifying the proteins that associate with RAS oncoproteins has great potential, not only to elucidate how these mutant proteins are regulated and signal but also to identify potential therapeutic targets. Here we describe a detailed protocol to employ proximity labeling by the BioID methodology, which has the advantage of capturing weak or transient interactions, to identify in an unbiased manner those proteins within the immediate vicinity of oncogenic RAS proteins.
鉴定与 RAS 癌蛋白相互作用的蛋白质具有很大的潜力，不仅可以阐明这些突变蛋白是如何被调控和传递信号的，还可以鉴定潜在的治疗靶点。在这里，我们描述了一种详细的方案，即利用 BioID 方法进行邻近标记，该方法具有捕获弱或瞬时相互作用的优势，以无偏倚的方式鉴定致癌 RAS 蛋白附近的那些蛋白质。
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