利用邻近依赖性生物素鉴定与蛋白质组学相结合的方法绘制酵母线粒体中的蛋白质网络。

Department of Biochemistry and Biophysics, Stockholm University, Stockholm 10691, Sweden.

Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Gothenburg 40530, Sweden.

STAR Protoc. 2020 Dec 15;1(3):100219. doi: 10.1016/j.xpro.2020.100219. eCollection 2020 Dec 18.

Proximity-dependent biotin identification (BioID) permits biotinylation of proteins interacting directly, indirectly, or just localized in proximity of a protein of interest (bait). Here, we describe how BioID coupled to proteomics and network biology can be used to map protein proximities in yeast mitochondria, aiding in visualization of complex protein-protein interaction landscapes. For complete information on the use and execution of this protocol, please refer to Singh et al., 2020.

邻近依赖性生物素鉴定（BioID）可使与研究目的蛋白（诱饵）直接、间接或仅在邻近位置相互作用的蛋白发生生物素化。在此，我们将描述如何将 BioID 与蛋白质组学和网络生物学相结合，用于绘制酵母线粒体中的蛋白质邻近关系，以帮助可视化复杂的蛋白质-蛋白质相互作用图谱。有关该方案使用和执行的完整信息，请参考 Singh 等人，2020。