Department of Parasitology and Tropical Medicine, School of Medicine, Pusan National University, Yangsan-si, Rep. of Korea.
Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan-si, Rep. of Korea.
Parasite Immunol. 2021 Sep;43(9):e12843. doi: 10.1111/pim.12843. Epub 2021 May 28.
Helminth infection typically induces a Th2 inflammatory response that is characterized by eosinophilia, high levels of IgE and mast cells. LTB4 is generated from innate immune cells, such as neutrophils, macrophages and mast cells, in response to a range of stimuli. It mainly acts on myeloid leukocytes, inducing the activation of integrins, adhesion to endothelium walls, and chemotaxis.
The objective of the present study was to determine the role of the LTB4 receptor in Trichinella spiralis expulsion. We treated mice with the LTB4 receptor antagonist before infection with T. spiralis. We observed that the number of mast cells and worm infection decreased following treatment with the BLT antagonist during the intestinal phase. We also demonstrated that blocking the LTB4 receptor inhibited neutrophil and eosinophil infiltration.
Further studies are required to investigate the specific mechanism of mast cell number decrease and worm infection and the in vitro interactions between LTB4 and worm expulsion.
寄生虫感染通常会诱导 Th2 炎症反应，其特征是嗜酸性粒细胞增多、IgE 和肥大细胞水平升高。LTB4 由中性粒细胞、巨噬细胞和肥大细胞等先天免疫细胞在一系列刺激下产生。它主要作用于髓样白细胞，诱导整合素的激活、与内皮细胞壁的黏附以及趋化性。
本研究旨在确定 LTB4 受体在旋毛虫排出中的作用。我们在感染旋毛虫前用 LTB4 受体拮抗剂处理小鼠。我们观察到，在肠道阶段用 BLT 拮抗剂治疗后，肥大细胞数量和虫体感染减少。我们还表明，阻断 LTB4 受体抑制了中性粒细胞和嗜酸性粒细胞的浸润。
需要进一步研究以探讨肥大细胞数量减少和虫体感染的具体机制，以及 LTB4 与虫体排出之间的体外相互作用。
