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球孢白僵菌对埃及伊蚊幼虫期的迟发性影响(双翅目:蚊科)。

Late effects of Beauveria bassiana on larval stages of Aedes aegypti Linneo‎, ‎1762‎ (Diptera: Culicidae).

机构信息

Universidad Autónoma de Nuevo León, Instituto de Biotecnología, Facultad de Ciencias Biológicas, San Nicolás de los Garza, N.L. México.

Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Laboratorio de Medicina de Conservación, Reynosa, Tamaulipas, México.

出版信息

Braz J Biol. 2021 May 10;82:e237789. doi: 10.1590/1519-6984.237789. eCollection 2021.

DOI:10.1590/1519-6984.237789
PMID:33978078
Abstract

Aedes aegypti is a culicide that has gained relevance over the years due to its ability to transmit various viruses that cause diseases in humans that all the years cause high mortality rates in the world population. The main problem is that Ae. aegypti has managed to establish and maintain a close relationship with humans and their habitat, which is why the search for alternatives to control vector populations becomes imperative. The objective of the present work was to study the effects of two Beauveria bassiana strains on Aedes aegypti. Third instar larvae of Ae. aegypti in 250 mL plastic containers were inoculated with the GHA and NB3 strains at different concentrations (1.5 × 104, 1.5× 105, 1.5 × 106 and 1.5 × 107 conidia/mL). The NB3 strain presented highest mortality values ​​with 63% in the highest concentration i.e., 1.5 × 107, while for the GHA strain the highest mortality value was 30.7% at the same concentration. The results showed significant difference in mortality with respect to the strain and days post treatment (P = 0.0001), but not with respect to the conidial concentration (P = 0.634). The average mortality of larvae per day for the NB3 for different concentrations ranged from 20 to 25 larvae per day, while for the GHA daily mortality ranged from 5 to 12 larvae. In post-treatment mortality, the highest mortality was recorded in the third stage larvae for the NB3, while for GHA the highest percentage mortality was observed in individuals who managed to reach the adult state. The findings of the current research depicted the noteworthy role of B. bassiana for the management of an important vector of human disease.

摘要

埃及伊蚊是一种半翅目昆虫,近年来由于其传播各种病毒的能力而引起关注,这些病毒会导致人类患病,多年来导致世界人口死亡率居高不下。主要问题是埃及伊蚊已经成功地与人类及其栖息地建立并维持了密切的关系,这就是为什么寻找控制病媒种群的替代方法变得至关重要的原因。本研究的目的是研究两种球孢白僵菌菌株对埃及伊蚊的影响。在 250 mL 塑料容器中,用 GHA 和 NB3 菌株以不同浓度(1.5×104、1.5×105、1.5×106 和 1.5×107 分生孢子/mL)接种三龄幼虫。NB3 菌株在最高浓度(即 1.5×107)下表现出最高的死亡率,为 63%,而 GHA 菌株在相同浓度下的最高死亡率为 30.7%。结果表明,死亡率与菌株和处理后天数之间存在显著差异(P=0.0001),但与分生孢子浓度无关(P=0.634)。不同浓度下 NB3 菌株的幼虫平均日死亡率范围为每天 20 到 25 头幼虫,而 GHA 菌株的日死亡率范围为每天 5 到 12 头幼虫。在处理后死亡率方面,NB3 对第三龄幼虫的死亡率最高,而 GHA 对达到成虫状态的个体的死亡率最高。本研究的结果表明,球孢白僵菌在人类疾病的重要病媒管理中具有重要作用。

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